Salmon keratocyte DIC microscopy videos (doi:10.18710/Q57B06)

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Part 2: Study Description
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Document Description

Citation

Title:

Salmon keratocyte DIC microscopy videos

Identification Number:

doi:10.18710/Q57B06

Distributor:

DataverseNO

Date of Distribution:

2021-11-26

Version:

1

Bibliographic Citation:

Opstad, Ida S.; Wolfson, Deanna L., 2021, "Salmon keratocyte DIC microscopy videos", https://doi.org/10.18710/Q57B06, DataverseNO, V1

Study Description

Citation

Title:

Salmon keratocyte DIC microscopy videos

Subtitle:

Dynamics of primary cells from Atlantic salmon scales acquired using differential interference contrast microscopy at room temperature

Identification Number:

doi:10.18710/Q57B06

Authoring Entity:

Opstad, Ida S. (UiT The Arctic University of Norway)

Wolfson, Deanna L. (UiT The Arctic University of Norway)

Other identifications and acknowledgements:

Wolfson, Deanna

Other identifications and acknowledgements:

Dalmo, Roy Ambli

Other identifications and acknowledgements:

Seternes, Tore

Other identifications and acknowledgements:

Agarwal, Krishna

Other identifications and acknowledgements:

Ahluwalia, Balpreet

Producer:

UiT The Arctic University of Norway

Grant Number:

Tematiske satsinger (Virtual Stain; 2061348)

Grant Number:

301401

Grant Number:

Video microscopy of salmon cells, disease progression and defence mechanisms

Distributor:

DataverseNO

Distributor:

UiT The Arctic University of Norway

Access Authority:

Opstad, Ida S.

Depositor:

Opstad, Ida Sundvor

Date of Deposit:

2021-10-20

Series Name:

Video microscopy of salmon cells

Series Information:

This is the first dataset in a planned series of microscopy time-lapse data of fish cells.

Holdings Information:

https://doi.org/10.18710/Q57B06

Study Scope

Keywords:

Medicine, Health and Life Sciences, Salmon, keratocyte, scale, live-cell, label-free, room temperature, differential interference contrast, DIC

Abstract:

Series of differential interference contrast microscopy (DIC) videos of primary keratocytes from Atlantic salmon scales acquired at room temperature (RT). The salmon from Kårvika (Tromsø Aquaculture Research Station) was about 1 kg +- 0.4 kg of either sex or smolt (about 10 to 15 cm long). The triploid salmon was > 1 kg and from a fish farm at Senja. The fish and cells were untreated. Unless specifically mentioned, the videos were acquired as single panels (field-of-view (FOV) 668 µm^2) 5 seconds apart for up to 20 hours, or larger areas (multiple panels (typically 4 by 4) of a combined FOV 2.7 mm^2) 2 minutes apart for up to 24 hours. The aim of the study was to investigate salmon skin cell dynamics, which are important to the innate immunity of fish.

Date of Collection:

2020-08-05-2021-05-06

Country:

Norway

Geographic Coverage:

Troms, Tromsø, Kårvika

Geographic Unit(s):

village

Kind of Data:

Experimental

Methodology and Processing

Sources Statement

Data Access

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