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Part 1: Document Description
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Citation |
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Title: |
Scripts and data for: Downregulation of the bacterial protein biosynthesis machinery in response to weeks, years, and decades of soil warming |
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Identification Number: |
doi:10.18710/HZBZQE |
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Distributor: |
DataverseNO |
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Date of Distribution: |
2021-11-26 |
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Version: |
1 |
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Bibliographic Citation: |
Söllinger, Andrea, 2021, "Scripts and data for: Downregulation of the bacterial protein biosynthesis machinery in response to weeks, years, and decades of soil warming", https://doi.org/10.18710/HZBZQE, DataverseNO, V1 |
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Citation |
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Title: |
Scripts and data for: Downregulation of the bacterial protein biosynthesis machinery in response to weeks, years, and decades of soil warming |
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Identification Number: |
doi:10.18710/HZBZQE |
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Authoring Entity: |
Söllinger, Andrea (UiT The Arctic University of Norway) |
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Producer: |
UiT The Arctic University of Norway |
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Software used in Production: |
R |
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Distributor: |
DataverseNO |
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Distributor: |
UiT The Arctic University of Norway |
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Access Authority: |
Söllinger, Andrea |
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Depositor: |
Söllinger, Andrea |
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Date of Deposit: |
2021-11-25 |
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Holdings Information: |
https://doi.org/10.18710/HZBZQE |
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Study Scope |
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Keywords: |
Earth and Environmental Sciences, Medicine, Health and Life Sciences, metatranscriptomics, ForHot, long-term soil warming, protein biosynthesis, differential gene expression, ribosome, soil microbiology |
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Abstract: |
This dataset contains all data files and R Markdown documents necessary to evaluate and repeat analyses presented in the manuscript: "Downregulation of the bacterial protein biosynthesis machinery in response to weeks, years, and decades of soil warming" |
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The study provides a first in-depth functional analysis of microbial responses to medium- (8 y) and long-term (>50 y) soil warming using a metatranscriptomics approach, i.e., high-throughput shotgun-sequencing of total RNA. Key finding: Soil warming induces a decrease of bacterial protein synthesis complexes (i.e. ribosomes), promoting high rates of growth and carbon dioxide production. |
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It is recommended to execute the R Markdown files in their given order (file numbers) to follow and re-create key figures and intermediate data files. |
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Please see related publication for additional details. |
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Time Period: |
2016-07-16-2021-11-25 |
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Country: |
Iceland |
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Geographic Coverage: |
Hveragerði |
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Kind of Data: |
metatranscriptomics data and context data |
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Kind of Data: |
R code |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Title: |
Söllinger, Andrea, Joana Séneca, Mathilde Borg Dahl, Liabo L. Motleleng, Judith Prommer, Erik Verbruggen, Bjarni D. Sigurdsson, et al. 2022. ‘Down-Regulation of the Bacterial Protein Biosynthesis Machinery in Response to Weeks, Years, and Decades of Soil Warming’. Science Advances 8 (12): eabm3230. https://doi.org/10.1126/sciadv.abm3230. |
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Identification Number: |
10.1126/sciadv.abm3230 |
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Bibliographic Citation: |
Söllinger, Andrea, Joana Séneca, Mathilde Borg Dahl, Liabo L. Motleleng, Judith Prommer, Erik Verbruggen, Bjarni D. Sigurdsson, et al. 2022. ‘Down-Regulation of the Bacterial Protein Biosynthesis Machinery in Response to Weeks, Years, and Decades of Soil Warming’. Science Advances 8 (12): eabm3230. https://doi.org/10.1126/sciadv.abm3230. |
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Label: |
000_README.txt |
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Text: |
README file containing a description of all different file types in this dataset |
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Notes: |
text/plain |
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001_metadata.txt |
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Notes: |
text/plain |
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001_metadata_and_Fig1.Rmd |
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Notes: |
application/octet-stream |
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002a_KO_tax_table_raw.txt |
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Notes: |
text/plain |
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002b_KEGG_mapping_July2019.txt |
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Notes: |
text/plain |
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002c_tax_table_raw.txt |
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Notes: |
text/plain |
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002_data_preparation.Rmd |
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Notes: |
application/octet-stream |
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003a_KO_tax_nff.txt |
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Notes: |
text/plain |
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003b_tax_nf_inkl_Vfam.txt |
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Notes: |
text/plain |
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003_KEGGOverview.Rmd |
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Notes: |
application/octet-stream |
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004_KEGG3_heapmaps_andMore.Rmd |
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Notes: |
application/octet-stream |
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005a_DGE_Analysis_AvsE.txt |
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Notes: |
text/plain |
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005b_DGE_Analysis_MTW_AvsE.txt |
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Notes: |
text/plain |
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005c_DGE_Analysis_LTW_AvsE.txt |
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Notes: |
text/plain |
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005_DGE_analysis_KEGG_edgeR.Rmd |
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Notes: |
application/octet-stream |
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005_enzyme_mapping_file.txt |
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Notes: |
text/plain |
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006a_proteinBS_oxidP_mapping_file.txt |
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Notes: |
text/plain |
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006b_DNA_repl_ftsZ_mapping_file.txt |
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Notes: |
text/plain |
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006_ProtBS_OxidP_Repl_DGE_details.Rmd |
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Notes: |
application/octet-stream |
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007_KEGG_mapper.Rmd |
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Notes: |
application/octet-stream |
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008_DGE_pattern_analysis_tax_edgeR.Rmd |
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Notes: |
application/octet-stream |
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009_SiteSpecific_DGE_pattern_analysis_tax_edgeR.Rmd |
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Notes: |
application/octet-stream |
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010_hypothesisTesting_october2020.Rmd |
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Notes: |
application/octet-stream |
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010_incubation_experiment_2020.txt |
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Notes: |
text/plain |
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010_RNA_2016_vs_2020.txt |
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Notes: |
text/plain |