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Part 1: Document Description
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Citation |
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Title: |
The proteome and secretome of rat liver sinusoidal endothelial cells in early primary culture, and effects of dexamethasone |
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Identification Number: |
doi:10.18710/AAJYOW |
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Distributor: |
DataverseNO |
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Date of Distribution: |
2021-05-03 |
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Version: |
3 |
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Bibliographic Citation: |
Bhandari, Sabin, 2021, "The proteome and secretome of rat liver sinusoidal endothelial cells in early primary culture, and effects of dexamethasone", https://doi.org/10.18710/AAJYOW, DataverseNO, V3 |
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Citation |
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Title: |
The proteome and secretome of rat liver sinusoidal endothelial cells in early primary culture, and effects of dexamethasone |
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Identification Number: |
doi:10.18710/AAJYOW |
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Authoring Entity: |
Bhandari, Sabin (UiT The Arctic University of Norway) |
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Other identifications and acknowledgements: |
Bruun, Jack-Ansgar |
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Other identifications and acknowledgements: |
Bhandari, Sabin |
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Other identifications and acknowledgements: |
UiT The Arctic University of Norway |
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Other identifications and acknowledgements: |
Sørensen, Karen |
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Other identifications and acknowledgements: |
Li, Ruomei |
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Other identifications and acknowledgements: |
Martinez, Inigo |
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Other identifications and acknowledgements: |
Urbarova, Ilona |
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Other identifications and acknowledgements: |
Smedsrød, Bård |
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Other identifications and acknowledgements: |
Simon-Santamaria, Jaione |
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Other identifications and acknowledgements: |
Vascular Biology Research Group (VBRG) |
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Other identifications and acknowledgements: |
UiT The Arctic University of Norway |
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Producer: |
UiT The Arctic University of Norway |
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Date of Production: |
2016-06-01 |
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Distributor: |
DataverseNO |
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Distributor: |
UiT The Arctic University of Norway |
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Access Authority: |
Sørensen, Karen Kristine |
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Depositor: |
Bhandari, Sabin |
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Date of Deposit: |
2021-04-29 |
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Holdings Information: |
https://doi.org/10.18710/AAJYOW |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, Rats, Liver, Proteome, Proteomics, Dexamethasone |
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Abstract: |
LSECs from male Sprague Dawley rats were cultured on collagen type-I in 5% oxygen atmosphere in DMEM with serum-free supplements for 2-24h. Quantitative proteomics using a tandem mass tag (TMT)6-plex strategy on Q-Exactive mass spectrometer. The worksheet contains the normalized reporter intensities for the 3 biological replicates for 5 samples representing 2h control, 24h control, 24h+IL-1beta, 24h + Dex, 24h + Dex and IL-1 beta |
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Date of Collection: |
2016-03-01- |
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Kind of Data: |
TMT-Sixplex labelled proteomic data |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Notes: |
Only after publication |
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Other Study Description Materials |
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Related Studies |
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The mass spectrometry proteomics raw data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD029241. Available at: https://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD029241 |
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The whole processed proteome is included in Supporting Information (S1 Dataset) and paper. Available at: https://doi.org/10.1371/journal.pone.0273843 |
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Related Publications |
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Citation |
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Title: |
Li R, Bhandari S, Martinez-Zubiaurre I, Bruun J-A, Urbarova I, Smedsrød B, et al. (2022) Changes in the proteome and secretome of rat liver sinusoidal endothelial cells during early primary culture and effects of dexamethasone. PLoS ONE 17(9): e0273843. https://doi.org/10.1371/journal.pone.0273843, |
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Identification Number: |
10.1371/journal.pone.0273843 |
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Bibliographic Citation: |
Li R, Bhandari S, Martinez-Zubiaurre I, Bruun J-A, Urbarova I, Smedsrød B, et al. (2022) Changes in the proteome and secretome of rat liver sinusoidal endothelial cells during early primary culture and effects of dexamethasone. PLoS ONE 17(9): e0273843. https://doi.org/10.1371/journal.pone.0273843, |
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Label: |
00_README_Rat_LSEC_proteome.txt |
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Notes: |
text/plain |
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Label: |
Rat_LSEC_cell_associated_processed_6plexTMT.txt |
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Notes: |
text/plain |
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Label: |
Rat_LSEC_supernatant_TMT6plex_proteomic .txt |
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Notes: |
text/plain |