The proteome and secretome of rat liver sinusoidal endothelial cells in early primary culture, and effects of dexamethasonedoi:10.18710/AAJYOWDataverseNO2021-05-033Bhandari, Sabin, 2021, "The proteome and secretome of rat liver sinusoidal endothelial cells in early primary culture, and effects of dexamethasone", https://doi.org/10.18710/AAJYOW, DataverseNO, V3The proteome and secretome of rat liver sinusoidal endothelial cells in early primary culture, and effects of dexamethasonedoi:10.18710/AAJYOWBhandari, SabinBruun, Jack-AnsgarBhandari, SabinUiT The Arctic University of NorwaySørensen, KarenLi, RuomeiMartinez, InigoUrbarova, IlonaSmedsrød, BårdSimon-Santamaria, JaioneVascular Biology Research Group (VBRG)UiT The Arctic University of NorwayUiT The Arctic University of Norway2016-06-01Proteomics and Metabolomics Core Facility (PRiME), UiT The Arctic University of NorwayDataverseNOUiT The Arctic University of NorwaySørensen, Karen KristineBhandari, Sabin2021-04-29Medicine, Health and Life SciencesRatsLiverProteomeProteomicsDexamethasoneLSECs from male Sprague Dawley rats were cultured on collagen type-I in 5% oxygen atmosphere in DMEM with serum-free supplements for 2-24h. Quantitative proteomics using a tandem mass tag (TMT)6-plex strategy on Q-Exactive mass spectrometer. The worksheet contains the normalized reporter intensities for the 3 biological replicates for 5 samples representing 2h control, 24h control, 24h+IL-1beta, 24h + Dex, 24h + Dex and IL-1 beta2016-03-01TMT-Sixplex labelled proteomic dataOnly after publicationThe mass spectrometry proteomics raw data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD029241.
Available at: https://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD029241The whole processed proteome is included in Supporting Information (S1 Dataset) and paper. Available at: https://doi.org/10.1371/journal.pone.0273843Li R, Bhandari S, Martinez-Zubiaurre I, Bruun J-A, Urbarova I, Smedsrød B, et al. (2022) Changes in the proteome and secretome of rat liver sinusoidal endothelial cells during early primary culture and effects of dexamethasone. PLoS ONE 17(9): e0273843. https://doi.org/10.1371/journal.pone.0273843,10.1371/journal.pone.0273843Li R, Bhandari S, Martinez-Zubiaurre I, Bruun J-A, Urbarova I, Smedsrød B, et al. (2022) Changes in the proteome and secretome of rat liver sinusoidal endothelial cells during early primary culture and effects of dexamethasone. PLoS ONE 17(9): e0273843. https://doi.org/10.1371/journal.pone.0273843,00_README_Rat_LSEC_proteome.txttext/plainRat_LSEC_cell_associated_processed_6plexTMT.txttext/plainRat_LSEC_supernatant_TMT6plex_proteomic .txttext/plain