Scripts and data for: Downregulation of the bacterial protein biosynthesis machinery in response to weeks, years, and decades of soil warmingdoi:10.18710/HZBZQEDataverseNO2021-11-261Söllinger, Andrea, 2021, "Scripts and data for: Downregulation of the bacterial protein biosynthesis machinery in response to weeks, years, and decades of soil warming", https://doi.org/10.18710/HZBZQE, DataverseNO, V1Scripts and data for: Downregulation of the bacterial protein biosynthesis machinery in response to weeks, years, and decades of soil warmingdoi:10.18710/HZBZQESöllinger, AndreaUiT The Arctic University of NorwayRDataverseNOUiT The Arctic University of NorwaySöllinger, AndreaSöllinger, Andrea2021-11-25Earth and Environmental SciencesMedicine, Health and Life SciencesmetatranscriptomicsForHotlong-term soil warmingprotein biosynthesisdifferential gene expressionribosomesoil microbiologyThis dataset contains all data files and R Markdown documents necessary to evaluate and repeat analyses presented in the manuscript: "Downregulation of the bacterial protein biosynthesis machinery in response to weeks, years, and decades of soil warming"The study provides a first in-depth functional analysis of microbial responses to medium- (8 y) and long-term (>50 y) soil warming using a metatranscriptomics approach, i.e., high-throughput shotgun-sequencing of total RNA.
Key finding: Soil warming induces a decrease of bacterial protein synthesis complexes (i.e. ribosomes), promoting high rates of growth and carbon dioxide production.It is recommended to execute the R Markdown files in their given order (file numbers) to follow and re-create key figures and intermediate data files.Please see related publication for additional details.2016-07-162021-11-25IcelandHveragerðimetatranscriptomics data and context dataR codeSöllinger, Andrea, Joana Séneca, Mathilde Borg Dahl, Liabo L. Motleleng, Judith Prommer, Erik Verbruggen, Bjarni D. Sigurdsson, et al. 2022. ‘Down-Regulation of the Bacterial Protein Biosynthesis Machinery in Response to Weeks, Years, and Decades of Soil Warming’. Science Advances 8 (12): eabm3230. https://doi.org/10.1126/sciadv.abm3230.10.1126/sciadv.abm3230Söllinger, Andrea, Joana Séneca, Mathilde Borg Dahl, Liabo L. Motleleng, Judith Prommer, Erik Verbruggen, Bjarni D. Sigurdsson, et al. 2022. ‘Down-Regulation of the Bacterial Protein Biosynthesis Machinery in Response to Weeks, Years, and Decades of Soil Warming’. Science Advances 8 (12): eabm3230. https://doi.org/10.1126/sciadv.abm3230.000_README.txtREADME file containing a description of all different file types in this datasettext/plain001_metadata.txttext/plain001_metadata_and_Fig1.Rmdapplication/octet-stream002a_KO_tax_table_raw.txttext/plain002b_KEGG_mapping_July2019.txttext/plain002c_tax_table_raw.txttext/plain002_data_preparation.Rmdapplication/octet-stream003a_KO_tax_nff.txttext/plain003b_tax_nf_inkl_Vfam.txttext/plain003_KEGGOverview.Rmdapplication/octet-stream004_KEGG3_heapmaps_andMore.Rmdapplication/octet-stream005a_DGE_Analysis_AvsE.txttext/plain005b_DGE_Analysis_MTW_AvsE.txttext/plain005c_DGE_Analysis_LTW_AvsE.txttext/plain005_DGE_analysis_KEGG_edgeR.Rmdapplication/octet-stream005_enzyme_mapping_file.txttext/plain006a_proteinBS_oxidP_mapping_file.txttext/plain006b_DNA_repl_ftsZ_mapping_file.txttext/plain006_ProtBS_OxidP_Repl_DGE_details.Rmdapplication/octet-stream007_KEGG_mapper.Rmdapplication/octet-stream008_DGE_pattern_analysis_tax_edgeR.Rmdapplication/octet-stream009_SiteSpecific_DGE_pattern_analysis_tax_edgeR.Rmdapplication/octet-stream010_hypothesisTesting_october2020.Rmdapplication/octet-stream010_incubation_experiment_2020.txttext/plain010_RNA_2016_vs_2020.txttext/plain