Replication Data for: Secretomic analysis of extracellular vesicles originating from polyomavirus-negative and polyomavirus-positive Merkel cell carcinoma cell lineshttps://doi.org/10.18710/USXCRSKonstantinell, Aelita Gloria VirginiaDataverseNO2019-05-062019-05-13T09:49:08ZThis dataset is about analyses of two Merkel cell polyomavirus (MCPyV)-negative and two MCPyV–positive Merkel cell lines secretomic proteins.</p>Abstract: Extracellular vesicles or exosomes constitute an evolutionarily conserved mechanism of intercellular signaling. Exosomes are gaining an increasing amount of attention due to their role
in pathologies, including malignancy, their importance as prognostic and diagnostic markers,
and their potential as a therapeutic tool. Merkel cell carcinoma (MCC) is an aggressive form of
skin cancer with a poor prognosis. Because an effective systemic treatment for this cancer type
is currently not available, an exosome-based therapy was proposed. However, comprehensive
secretome profiling has not been performed for MCC. To help unveil the putative contribution
of exosomes in MCC, we studied the protein content of MCC-derived exosomes. Since approximately
80% of all MCC cases contain Merkel cell polyomavirus (MCPyV), the secretomes of
two MCPyV-negative and two MCPyV-positive MCC cell lines were compared. We identified
with high confidence 164 exosome-derived proteins common for all four cell lines that were annotated
in ExoCarta and Vesiclepedia databases. These include proteins implicated in motility,
metastasis and tumor progression, such as integrins and tetraspanins, intracellular signaling
molecules, chaperones, proteasomal proteins, and translation factors. Additional virus-negative
and virus-positiveMCC cell lines should be examined to identify highly representative exosomal
proteins that may provide reliable prognostic and diagnostic biomarkers, as well as targets for
treatment in the future. Data are available via ProteomeXchange with identifier PXD004198.Medicine, Health and Life SciencesMerkel cell carcinomaMerkel cell polyomavirusExosomeProteomicsA. Konstantinell, et al. Secretomic analysis of extracellular vesicles originating from polyomavirus-negative and polyomavirus-positive Merkel cell carcinoma cell lines. Proteomics 2016, 16, 2587-2591., doi, 10.1002/pmic.201600223, https://doi.org/10.1002/pmic.2016002232019-05-06Konstantinell, Aelita Gloria Virginia2019-04-012015-08-012015-12-31Biomarkers Discovery: The Benefit of the Study Exosomes Originated from Merkel Cell Carcinoma Cell Lines. doi: 10.18710/KCW912Experimental dataCC0 1.0