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Part 1: Document Description
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Citation |
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Title: |
Salmon keratocyte DIC microscopy videos |
Identification Number: |
doi:10.18710/Q57B06 |
Distributor: |
DataverseNO |
Date of Distribution: |
2021-11-26 |
Version: |
1 |
Bibliographic Citation: |
Opstad, Ida S.; Wolfson, Deanna L., 2021, "Salmon keratocyte DIC microscopy videos", https://doi.org/10.18710/Q57B06, DataverseNO, V1 |
Citation |
|
Title: |
Salmon keratocyte DIC microscopy videos |
Subtitle: |
Dynamics of primary cells from Atlantic salmon scales acquired using differential interference contrast microscopy at room temperature |
Identification Number: |
doi:10.18710/Q57B06 |
Authoring Entity: |
Opstad, Ida S. (UiT The Arctic University of Norway) |
Wolfson, Deanna L. (UiT The Arctic University of Norway) |
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Other identifications and acknowledgements: |
Wolfson, Deanna |
Other identifications and acknowledgements: |
Dalmo, Roy Ambli |
Other identifications and acknowledgements: |
Seternes, Tore |
Other identifications and acknowledgements: |
Agarwal, Krishna |
Other identifications and acknowledgements: |
Ahluwalia, Balpreet |
Producer: |
UiT The Arctic University of Norway |
Grant Number: |
Tematiske satsinger (Virtual Stain; 2061348) |
Grant Number: |
301401 |
Grant Number: |
Video microscopy of salmon cells, disease progression and defence mechanisms |
Distributor: |
DataverseNO |
Distributor: |
UiT The Arctic University of Norway |
Access Authority: |
Opstad, Ida S. |
Depositor: |
Opstad, Ida Sundvor |
Date of Deposit: |
2021-10-20 |
Series Name: |
Video microscopy of salmon cells |
Series Information: |
This is the first dataset in a planned series of microscopy time-lapse data of fish cells. |
Holdings Information: |
https://doi.org/10.18710/Q57B06 |
Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, Salmon, keratocyte, scale, live-cell, label-free, room temperature, differential interference contrast, DIC |
Abstract: |
Series of differential interference contrast microscopy (DIC) videos of primary keratocytes from Atlantic salmon scales acquired at room temperature (RT). The salmon from Kårvika (Tromsø Aquaculture Research Station) was about 1 kg +- 0.4 kg of either sex or smolt (about 10 to 15 cm long). The triploid salmon was > 1 kg and from a fish farm at Senja. The fish and cells were untreated. Unless specifically mentioned, the videos were acquired as single panels (field-of-view (FOV) 668 µm^2) 5 seconds apart for up to 20 hours, or larger areas (multiple panels (typically 4 by 4) of a combined FOV 2.7 mm^2) 2 minutes apart for up to 24 hours. The aim of the study was to investigate salmon skin cell dynamics, which are important to the innate immunity of fish. |
Date of Collection: |
2020-08-05-2021-05-06 |
Country: |
Norway |
Geographic Coverage: |
Troms, Tromsø, Kårvika |
Geographic Unit(s): |
village |
Kind of Data: |
Experimental |
Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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video/avi |
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03092020_kera_L15_day10_002_R3D.avi |
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video/x-msvideo |
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31082020_salmon_scale_L15Lday7_10x_DIC_5sTL_003_R3D.avi |
Notes: |
video/x-msvideo |
Label: |
salmon_scale_L15Lday2_10x_DIC_5sTL_002_R3D.avi |
Notes: |
video/x-msvideo |
Label: |
salmon_scale_L15Lday2_10x_DIC_5sTL_003_R3D.avi |
Notes: |
video/x-msvideo |
Label: |
salmon_scale_L15Lday4_10x_DIC_5sTL_001_R3D.avi |
Notes: |
video/x-msvideo |
Label: |
salmon_scale_L15Lday4_10x_DIC_5sTL_004_R3D.avi |
Notes: |
video/x-msvideo |
Label: |
salmon_scale_L15Lday7_10x_DIC_5sTL_002_R3D.avi |
Notes: |
video/x-msvideo |