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Part 1: Document Description
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Citation |
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Title: |
3DSIM data of mitochondria in the cardiomyoblast cell-line H9c2 adapted to either glucose or galactose |
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Identification Number: |
doi:10.18710/PDCLAS |
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Distributor: |
DataverseNO |
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Date of Distribution: |
2021-09-23 |
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Version: |
2 |
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Bibliographic Citation: |
Opstad, Ida S., 2021, "3DSIM data of mitochondria in the cardiomyoblast cell-line H9c2 adapted to either glucose or galactose", https://doi.org/10.18710/PDCLAS, DataverseNO, V2 |
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Citation |
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Title: |
3DSIM data of mitochondria in the cardiomyoblast cell-line H9c2 adapted to either glucose or galactose |
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Identification Number: |
doi:10.18710/PDCLAS |
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Authoring Entity: |
Opstad, Ida S. (UiT The Arctic University of Norway) |
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Other identifications and acknowledgements: |
Birgisdottir, Åsa Birna |
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Other identifications and acknowledgements: |
Myrmel, Truls |
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Other identifications and acknowledgements: |
Godtliebsen, Gustav |
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Other identifications and acknowledgements: |
Agarwal, Krishna |
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Other identifications and acknowledgements: |
Ahluwalia, Balpreet S. |
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Other identifications and acknowledgements: |
Sarre, Aili |
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Other identifications and acknowledgements: |
Conzett, Philipp |
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Producer: |
UiT The Arctic University of Norway |
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Date of Production: |
2021-06-28 |
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Grant Number: |
HNF1449-19 |
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Grant Number: |
VirtualStain; project number 2061348 |
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Distributor: |
DataverseNO |
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Distributor: |
UiT The Arctic University of Norway |
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Access Authority: |
Opstad, Ida S. |
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Depositor: |
Opstad, Ida Sundvor |
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Date of Deposit: |
2021-05-30 |
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Holdings Information: |
https://doi.org/10.18710/PDCLAS |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, 3DSIM, SIM, Structured illumination microscopy, super-resolution microscopy, live-cell, fixed-cell, mitochondria, mitochondrial nanotubes, MDVs, mitochondria-derived vesicles, mCherry-EGFP, mitochondrial outer membrane protein 25 transmembrane domain, OMP25-TM, stable expression, galactose adapted, H9c2, cardiomyoblasts, outer mitochondrial membrane, lysosomes, lysotracker deep red |
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Abstract: |
<p><b>ALTERNATIVE DOWNLOAD FROM FileSender: </b> To access the data via https with limited network capacity, click <a href="https://filesender.uninett.no/?s=download&token=8ab228ed-808b-452c-9a74-9b53ac91d5f4">here.</a></p> <p></p> <p>Series of three-dimensional structured illumination microscopy (3DSIM) of the rat cardiomyoblast cell-line H9c2 adapted to either glucose (GLU) or galactose (GAL). The outer mitochondrial membranes are labeled with the double tag mCherry-EGFP-OMP-25TM (dTag), i.e., both a red and green fluorescent protein variant. dTag can be used as a sensor for acidity (or lysosomal degradation), as the green fluorescence is lost sooner in an acidic environment than the red fluorescence. Where indicated, the samples are additionally labeled with LysoTracker Deep Red (LTDR). The aim of the study was to test the feasibility of multi-color 3DSIM for the examination of mitochondria-derived vesicles (MDVs) in both living and fixed cells.</p> |
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Date of Collection: |
2021-04-20-2021-05-11 |
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Country: |
Norway |
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Geographic Coverage: |
Troms, Tromsø |
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Kind of Data: |
Experimental |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Title: |
Opstad, I. S., Godtliebsen, G., Ahluwalia, B. S., Myrmel, T., Agarwal, K., & Birgisdottir, Å. B. (2022). Mitochondrial dynamics and quantification of mitochondria‐derived vesicles in cardiomyoblasts using structured illumination microscopy. Journal of Biophotonics, 15(2), e202100305. |
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Identification Number: |
10.1002/jbio.202100305 |
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Bibliographic Citation: |
Opstad, I. S., Godtliebsen, G., Ahluwalia, B. S., Myrmel, T., Agarwal, K., & Birgisdottir, Å. B. (2022). Mitochondrial dynamics and quantification of mitochondria‐derived vesicles in cardiomyoblasts using structured illumination microscopy. Journal of Biophotonics, 15(2), e202100305. |
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Citation |
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Title: |
Opstad, I. S., Godtliebsen, G., Ströhl, F., Myrmel, T., Ahluwalia, B. S., Agarwal, K., & Birgisdottir, Å. B. (2022). Three-dimensional structured illumination microscopy data of mitochondria and lysosomes in cardiomyoblasts under normal and galactose-adapted conditions. Scientific Data, 9(1), 1-7. |
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Identification Number: |
10.1038/s41597-022-01207-7 |
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Bibliographic Citation: |
Opstad, I. S., Godtliebsen, G., Ströhl, F., Myrmel, T., Ahluwalia, B. S., Agarwal, K., & Birgisdottir, Å. B. (2022). Three-dimensional structured illumination microscopy data of mitochondria and lysosomes in cardiomyoblasts under normal and galactose-adapted conditions. Scientific Data, 9(1), 1-7. |
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Label: |
00_FileOrganization.txt |
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Notes: |
text/plain |
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Label: |
00_ReadMe.txt |
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Notes: |
text/plain |
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Label: |
FixedGA_LTDR_GAL_3DSIM.zip |
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Notes: |
application/zip |
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FixedGA_LTDR_GAL_3DSIM_SIRlog.zip |
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Notes: |
application/zip |
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FixedGA_LTDR_GAL_Conventional.zip |
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application/zip |
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FixedGA_LTDR_GAL_RawSIM.zip |
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application/zip |
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FixedGA_LTDR_GAL_RawSIM_log.zip |
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application/zip |
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FixedGA_LTDR_GAL_SIMmaxPrj.zip |
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application/zip |
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FixedGA_LTDR_GLU_3DSIM.zip |
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application/zip |
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FixedGA_LTDR_GLU_3DSIM_SIRlog.zip |
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application/zip |
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FixedGA_LTDR_GLU_Conventional.zip |
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application/zip |
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FixedGA_LTDR_GLU_RawSIM.zip |
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Notes: |
application/zip |
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FixedGA_LTDR_GLU_RawSIM_log.zip |
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application/zip |
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FixedGA_LTDR_GLU_SIMmaxPrj.zip |
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application/zip |
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FixedGA_LTDR_ParameterFiles.zip |
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application/zip |
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FixedPFA_3DSIM.zip |
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application/zip |
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FixedPFA_3DSIM_SIRlog.zip |
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application/zip |
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FixedPFA_Conventional.zip |
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application/zip |
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FixedPFA_ParameterFiles.zip |
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application/zip |
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FixedPFA_RawSIM.zip |
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application/zip |
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FixedPFA_RawSIM_log.zip |
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application/zip |
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FixedPFA_SIMmaxPrj.zip |
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application/zip |
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LiveCell_3DSIM_Part_1.zip |
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application/zip |
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LiveCell_3DSIM_Part_2.zip |
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application/zip |
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LiveCell_3DSIM_Part_3.zip |
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application/zip |
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LiveCell_3DSIM_SIRlog.zip |
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application/zip |
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LiveCell_AVI_movies.zip |
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application/zip |
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LiveCell_Conventional.zip |
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application/zip |
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LiveCell_ParameterFiles.zip |
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application/zip |
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LiveCell_RawSIM_Part_1.zip |
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application/zip |
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LiveCell_RawSIM_Part_2.zip |
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application/zip |
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LiveCell_RawSIM_Part_3.zip |
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application/zip |
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LiveCell_RawSIM_Part_4.zip |
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application/zip |
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LiveCell_RawSIM_Part_5.zip |
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application/zip |
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LiveCell_RawSIM_Part_6.zip |
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application/zip |
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LiveCell_RawSIM_Part_7.zip |
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application/zip |
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LiveCell_RawSIM_Part_8.zip |
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application/zip |
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LiveCell_RawSIM_log.zip |
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application/zip |
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LiveCell_SIMmaxPrj.zip |
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application/zip |
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LiveCell_LTDR_GAL_3DSIM.zip |
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application/zip |
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LiveCell_LTDR_GAL_3DSIM_SIRlog.zip |
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Notes: |
application/zip |
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LiveCell_LTDR_GAL_AVI.zip |
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application/zip |
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LiveCell_LTDR_GAL_Conventional.zip |
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application/zip |
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LiveCell_LTDR_GAL_RawSIM.zip |
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application/zip |
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LiveCell_LTDR_GAL_RawSIM_log.zip |
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application/zip |
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LiveCell_LTDR_GAL_SIMmaxPrj.zip |
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application/zip |
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LiveCell_LTDR_GLU_3DSIM.zip |
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application/zip |
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LiveCell_LTDR_GLU_3DSIM_SIRlog.zip |
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Notes: |
application/zip |
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LiveCell_LTDR_GLU_AVI.zip |
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application/zip |
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LiveCell_LTDR_GLU_Conventional.zip |
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application/zip |
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LiveCell_LTDR_GLU_RawSIM.zip |
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application/zip |
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LiveCell_LTDR_GLU_RawSIM_log.zip |
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application/zip |
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LiveCell_LTDR_GLU_SIMmaxPrj.zip |
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application/zip |
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LiveCell_LTDR_ParameterFiles.zip |
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application/zip |