Replication Data for: Advancing Quantification methods for Polyphenols in Brown Seaweeds - applying a Selective qNMR Method compared with the TPC Assaydoi:10.18710/VZHSWTDataverseNO2022-07-121Wekre, Marie Emilie; Jordheim, Monica; Brunvoll, Sondre Hellesen, 2022, "Replication Data for: Advancing Quantification methods for Polyphenols in Brown Seaweeds - applying a Selective qNMR Method compared with the TPC Assay", https://doi.org/10.18710/VZHSWT, DataverseNO, V1Replication Data for: Advancing Quantification methods for Polyphenols in Brown Seaweeds - applying a Selective qNMR Method compared with the TPC Assaydoi:10.18710/VZHSWTWekre, Marie EmilieJordheim, MonicaBrunvoll, Sondre HellesenWekre, Marie EmilieBrunvoll, Sondre HellesenJordheim, MonicaAlginor ASAUniversity of BergenBergenTopspinMestReNovaNFR297507DataverseNOUniversity of BergenJordheim, MonicaWekre, Marie Emilie2021-11-252022-07-07ChemistryTotal Phenolic ContentqNMRPolyphenolsLaminaria hyperboreaLaminaria digitataFucus vesiculosusSaccharina latissimaAscophyllum nodosumQuantificationTPCThese data belong to the comprehensive study of polyphenols in Laminaria hyperborea, Laminaria digitata, Saccharina latissima, Ascophyllum nodosum and Fucus vesiculosus performed in relation with the publication titled: "Advancing Quantification methods for Polyphenols in Brown Seaweeds - applying a Selective qNMR Method compared with the TPC Assay". The dataset contains 22 files, describing the quantitative content of polyphenols in the brown seaweeds Laminaria hyperborea, Laminaria digitata, Saccharina latissima, Ascophyllum nodosum and Fucus vesiculosus. The quantitative content was determined by Optimized Total Phenolic Content (TPC) assay and quantitative NMR (qNMR).Publication abstract:
Polyphenols are important components of seaweeds contributing to their potential use in food-, feed- and health applications. In this study, optimized methods of the Folin-Ciocalteu total phenolic content assay (TPC) and selective quantitative 1H NMR (qNMR), including 13C NMR characterization, were compared for selected brown seaweeds from sublittoral to intertidal zone (Laminaria hyperborea, Laminaria digitata, and Fucus vesiculosus). TPC and selective qNMR resulted in comparable quantities for the sublittoral growing Laminariaceae, with lower polyphenolic content (TPC: 0.6–0.7%; qNMR: 0.7–0.8%, GAE, DW). F. vesiculosus, found in more shallow waters with a higher polyphenolic production, yields a TPC quantification more than double the qNMR quantification (TPC: 3.7%; qNMR: 1.5% GAE, DW). This difference most likely reflects the lack of sensitivity in the TPC assay concerning species with interfering compounds and/or a higher polyphenolic content, and conceivably also a higher diversity of polyphenols (> 1 % of biomass). From the 13C NMR characterization, L. hyperborea, L. digitata, and F. vesiculosus all showed a larger proportion of phlorethol-like phlorotannins compared to fucol-type phlorotannins.2019-08-012022-03-01NorwayNorwayNorwayAustraliaNorwayVestlandTrollsøyBergenRogalandHaugesundRogaland Field 55EVestlandStoråkervikaBergenMelbourneVictoriaVestlandBergenEidsvågQuantitative dataExperimental dataWekre, ME, Hellesen Brunvoll, S, Jordheim, M. Advancing quantification methods for polyphenols in brown seaweeds—applying a selective qNMR method compared with the TPC assay. Phytochemical Analysis. 2022; 1- 12. doi:10.1002/pca.316210.1002/pca.3162Wekre, ME, Hellesen Brunvoll, S, Jordheim, M. Advancing quantification methods for polyphenols in brown seaweeds—applying a selective qNMR method compared with the TPC assay. Phytochemical Analysis. 2022; 1- 12. doi:10.1002/pca.3162A.Nodosum_S17_qNMR_calculations.txtThis data set presents quantification data from qNMR for Ascophyllym nodosum harvested in September 2017.text/plainA.Nodosum_S17_qNMR_rawdata.txtThis dataset contains the raw data used for quantification of Ascophyllym nodosum harvested in September 2017.text/plainF.vesiculosus_qNMR_calculations.txtThis data set presents quantification data from qNMR for Fucus vesiculosus. text/plainF.vesiculosus_qNMR_rawdata.txtThis dataset contains the raw data used for quantification of Fucus vesiculosus. text/plainL.digitata_qNMR_calculations.txtThis data set presents quantification data from qNMR for Laminaria digitata. text/plainL.digitata_qNMR_rawdata.txtThis dataset contains the raw data used for quantification of Laminaria digitata. text/plainL.hyperborea_A21_qNMR_calculations.txtThis data set presents quantification data from qNMR for Laminaria hyperborea harvested in August 2021. text/plainL.hyperborea_A21_qNMR_rawdata.txtThis dataset contains the raw data used for quantification of Laminaria hyperborea harvested in August 2021. text/plainL.hyperborea_M20_qNMR_calculations.txtThis data set presents quantification data from qNMR for Laminaria hyperborea harvested in March 2020. text/plainL.hyperborea_M20_qNMR_rawdata.txtThis dataset contains the raw data used for quantification of Laminaria hyperborea harvested in March 2020. text/plainL.hyperborea_S20_qNMR_calculations.txtThis data set presents quantification data from qNMR for Laminaria hyperborea harvested in September 2020. text/plainL.hyperborea_S20_qNMR_rawdata.txtThis dataset contains the raw data used for quantification of Laminaria hyperborea harvested in September 2020. text/plainL.Saccharina_qNMR_calculations.txtThis data set presents quantification data from qNMR for Fucus vesiculosus.text/plainL.Saccharina_qNMR_rawdata.txtThis dataset contains the raw data used for quantification of Laminaria saccharina.text/plainqNMR_standardvalidation.txtThis dataset describes the quantification of two standards, gallic acid and phloroglucinol, to validate the qNMR method. text/plainREADME.txtReadme file describing datasets. text/plainTPC_gallicacid_calculations.txtContains the quantifications of all extracts using TPC with the gallic acid calibration curve. text/plainTPC_gallicacid_calibrationcurve.txtDataset describing raw data for the calibration curve of gallic acid used in TPC quantifications. Also describing the dry weights of the extracts used for calculation. text/plainTPC_phloroglucinol_calculations.txtContains the quantifications of all extracts using TPC with the phloroglucinol calibration curve.text/plainTPC_phloroglucinol_calibrationcurve.txtDataset describing raw data for the calibration curve of phloroglucinol used in TPC quantifications. Also describing the dry weights of the extracts used for calculation. text/plain