Replication Data for: Using near-infrared reflectance spectroscopy (NIRS) to predict carbon and nitrogen stable isotope composition in animal tissuesdoi:10.18710/5PX1GJDataverseNO2021-07-091Ancin-Murguzur, Francisco Javier, 2021, "Replication Data for: Using near-infrared reflectance spectroscopy (NIRS) to predict carbon and nitrogen stable isotope composition in animal tissues", https://doi.org/10.18710/5PX1GJ, DataverseNO, V1Replication Data for: Using near-infrared reflectance spectroscopy (NIRS) to predict carbon and nitrogen stable isotope composition in animal tissuesdoi:10.18710/5PX1GJAncin-Murguzur, Francisco JavierTarroux, ArnaudUiT The Arctic University of Norway2021-01-01TromsøR2011/70/8/KH/isDataverseNOUiT The Arctic University of NorwayAncin-Murguzur, Francisco JavierAncin Murguzur, Francisco Javier2021-02-05Earth and Environmental Sciencesnirsnear-infrared reflectance spectroscopyisotopebirdbloodred blood cells1. Stable isotopes analysis (SIA) of carbon and nitrogen provides valuable information about trophic interactions and animal feeding habits.
2. We used near-infrared reflectance spectroscopy (NIRS) and support vector machines (SVM) to develop a model for screening isotopic ratios of carbon and nitrogen (δ13C and δ15N) in samples from living animals. We applied this method on dried blood samples from birds previously analysed for δ13C and δ15N to test whether NIRS can be applied to accurately predict isotopic ratios.
3. Our results show a prediction accuracy of NIRS (R2>0.65, RMSEP<0.28) for both δ13C and δ15N, representing a 12% of the measurement range in this study.
4. Our study suggests that NIRS can provide a time- and cost-efficient method to evaluate stable isotope ratios of carbon and nitrogen when substantial differences in δ13C or δ15N are expected, such as when discriminating among different trophic levels in diet.2011-10-012014-03-01AntarcticaExperimentalAncin-Murguzur, F. J., Tarroux, A., Bråthen, K. A., Bustamante, P., & Descamps, S. (2021). Using near-infrared reflectance spectroscopy (NIRS) to estimate carbon and nitrogen stable isotope composition in animal tissues. Ecology and Evolution, 00, 1– 6.10.1002/ece3.7851Ancin-Murguzur, F. J., Tarroux, A., Bråthen, K. A., Bustamante, P., & Descamps, S. (2021). Using near-infrared reflectance spectroscopy (NIRS) to estimate carbon and nitrogen stable isotope composition in animal tissues. Ecology and Evolution, 00, 1– 6.00_README.txtREADME filetext/plainScript.RThe "Script.R" file contains all the required R commands to develop the main model described in the related publicationtype/x-r-syntaxSpectra.txtThe "Spectra.txt" file contains the reference spectral dataset, with columns named with their corresponding wavelength number (X350 to X2450) and the sample identifier ("Sample")text/plainValues.txtThe "Values.txt" file contains the reference chemical values for d13C, d15N, C, N and the C/N ratios (with equivalent column names) as well as the sample identifiertext/plain