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Part 1: Document Description
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Citation |
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Title: |
Replication data for: Following the Fate of Dye-Containing Liposomes in Vitro |
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Identification Number: |
doi:10.18710/5E8VUI |
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Distributor: |
DataverseNO |
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Date of Distribution: |
2020-10-13 |
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Version: |
1 |
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Bibliographic Citation: |
Cauzzo, Jennifer; Nystad, Mona; Holsæter, Ann Mari; Basnet, Purusotam; Skalko-Basnet, Natasa, 2020, "Replication data for: Following the Fate of Dye-Containing Liposomes in Vitro", https://doi.org/10.18710/5E8VUI, DataverseNO, V1 |
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Citation |
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Title: |
Replication data for: Following the Fate of Dye-Containing Liposomes in Vitro |
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Identification Number: |
doi:10.18710/5E8VUI |
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Authoring Entity: |
Cauzzo, Jennifer (UiT The Arctic University of Norway) |
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Nystad, Mona (UiT The Arctic University of Norway; University Hospital of North Norway) |
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Holsæter, Ann Mari (UiT The Arctic University of Norway) |
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Basnet, Purusotam (UiT The Arctic University of Norway; University Hospital of North Norway) |
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Skalko-Basnet, Natasa (UiT The Arctic University of Norway) |
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Other identifications and acknowledgements: |
Lyså, Roy Andre |
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Producer: |
UiT The Arctic University of Norway |
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Date of Production: |
2020-04-24 |
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Grant Number: |
766181 |
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Distributor: |
DataverseNO |
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Distributor: |
UiT The Arctic University of Norway |
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Access Authority: |
Skalko-Basnet, Natasa |
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Depositor: |
Cauzzo, Jennifer |
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Date of Deposit: |
2020-09-28 |
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Holdings Information: |
https://doi.org/10.18710/5E8VUI |
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Study Scope |
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Keywords: |
Chemistry, Medicine, Health and Life Sciences, Liposomes, Nanomedicine, Fluorescent Dyes |
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Abstract: |
This dataset contains the experimental work associated to the publication "Following the Fate of Dye-Containing Liposomes In Vitro" Int. J. Mol. Sci. 2020, 21(14), 4847; https://doi.org/10.3390/ijms21144847. <p> The rather limited success of translation from basic research to clinical application has been highlighted as a major issue in the nanomedicine field. To identify the factors influencing the applicability of nanosystems as drug carriers and potential nanomedicine, we focused on following their fate through fluorescence-based assays, namely flow cytometry and imaging. These methods are often used to follow the nanocarrier internalization and targeting; however, the validity of the obtained results strictly depends on how much the nanosystem’s fate can be inferred from the fate of fluorescent dyes. To evaluate the parameters that affect the physicochemical and biological stability of the labeled nanosystems, we studied the versatility of two lipid dyes, TopFluor®-PC and Cy5-DSPE, in conventional liposomes utilizing well-defined in vitro assays. Our results suggest that the dye can affect the major characteristics of the system, such as vesicle size and zeta-potential. However, a nanocarrier can also affect the dye properties. Medium, temperature, time, fluorophore localization and its concentration, as well as their interplay, affect the outcome of tracing experiments. Therefore, an in-depth characterization of the labeled nanosystem should be fundamental to understand the conditions that validate the results within the screening process in optimization of nanocarrier. </p> |
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This dataset is a collection of laboratory experiments for the characterization of the physicochemical properties of fluorescently labeled liposomes, as well as their behavior in biological environment. The experiments include: size stability (NICOMP photon correlation spectroscopy), zeta-potential stability (Zetasizer), fluorescence stability (SPARK spettrofluorimeter), biological effect (NO production on LPS-induced macrophages, cell proliferation on macrophages and keratinocytes), fluorescence tracing (Flow cytometry and imaging). |
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Date of Collection: |
2019-01-07-2019-11-22 |
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Kind of Data: |
Experimental data |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Title: |
Cauzzo, J.; Nystad, M.; Holsæter, A.M.; Basnet, P.; Škalko-Basnet, N. Following the Fate of Dye-Containing Liposomes In Vitro. Int. J. Mol. Sci. 2020, 21, 4847. |
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Identification Number: |
10.3390/ijms21144847 |
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Bibliographic Citation: |
Cauzzo, J.; Nystad, M.; Holsæter, A.M.; Basnet, P.; Škalko-Basnet, N. Following the Fate of Dye-Containing Liposomes In Vitro. Int. J. Mol. Sci. 2020, 21, 4847. |
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