Supplementary dataset for "Characterization of Human Stem Cell-Derived Hepatic Stellate Cells and Liver Sinusoidal Endothelial Cells During Extended in vitro Culture"

10.18710/4MJLC3 Wilhelmsen, Ingrid Ingrid Wilhelmsen https://orcid.org/0009-0009-5285-0932 University of Oslo Supplementary dataset for "Characterization of Human Stem Cell-Derived Hepatic Stellate Cells and Liver Sinusoidal Endothelial Cells During Extended in vitro Culture" DataverseNO 2023 Medicine, Health and Life Sciences Hepatic stellate cell (HSC) a-smooth muscle actin (a-sMA) Induced pluripotent stem cells Liver sinusoidal endothelial cell (LSEC) Induced pluripotent stem cell Confocal immunofluorescence microscopy University of Oslo University of Oslo Wilhelmsen, Ingrid Ingrid Wilhelmsen University of Oslo Krauss, Stefan Stefan Krauss Aizenshtadt, Aleksandra Aleksandra Aizenshtadt Combriat, Thomas Thomas Combriat Amirola Martinez, Mikel Mikel Amirola Martinez Stokowiec, Justyna Justyna Stokowiec Wang, Chencheng Chencheng Wang Wilhelmsen, Ingrid Ingrid Wilhelmsen 2023-07-06 2023-09-26 2023-09-28 2022-10-01/2022-12-15 Image data 10.3389/FBIOE.2023.1223737 10.18710/4MJLC3/L29PET 10.18710/4MJLC3/AHSSSX 10.18710/4MJLC3/RSYYO6 5019 8339605662 8331545949 text/plain application/zip application/zip 1.1 Creative Commons CC0 1.0 Universal Public Domain Dedication. The dataset contains confocal immunofluorescence images of human pluripotent stem cell-derived hepatic stellate cells (scHSCs) and human primary HSCs (pHSCs). In the homeostatic liver, HSCs exist in a quiescent state. Upon liver injury, HSCs differentiate into an activated, myofibroblast-like state. The activated HSCs are phenotypically characterized by the intracellular expression of alpha-smooth muscle actin (a-SMA). Transforming growth factor beta (TGF-b) is a potent HSC activator and is commonly used to assess the activation capacity of cultured HSCs. In this dataset, scHSCs and pHSCs were grown in vitro and stained with immunofluorescent labels for the HSC marker PDGFR-b as well as the activation-related proteins a-SMA and COL1a1. Image analysis of the dataset was performed to quantify a-SMA fluorescence intensity to assess the activation status of the HSCs in the presence and absence of TGF-b. An example script to analyze the data is provided on GitHub: https://github.com/ingridwilhelmsen/a-SMA_analysis/blob/main/a-SMA_analysis-Activation.ipynb ZEN Blue, 3.5.093.00003 The Research Council of Norway 262613 Health Region East (HSØ) 2021068