10.18710/USXCRS Konstantinell, Aelita Gloria Virginia Aelita Gloria Virginia Konstantinell UiT The Arctic University of Norway DataverseNO 2019 Medicine, Health and Life Sciences Merkel cell carcinoma Merkel cell polyomavirus Exosome Proteomics UiT The Arctic University of Norway UiT The Arctic University of Norway UiT The Arctic University of Norway Konstantinell, Aelita Gloria Virginia Aelita Gloria Virginia Konstantinell UiT The Arctic University of Norway 2019-04-01 2019-05-06 2019-05-13 2015-08-01/2015-12-31 Experimental data 10.1002/PMIC.201600223 10.18710/USXCRS/GYUFPQ 10.18710/USXCRS/IRUGPH 10.18710/USXCRS/GRUGKV 10.18710/USXCRS/R7F8QG 10.18710/USXCRS/HAIGG2 10.18710/USXCRS/YGAJUZ 10.18710/USXCRS/9JBLDY 10.18710/USXCRS/4KVUUX 10.18710/USXCRS/JRVCQY 10.18710/USXCRS/IKNWIB 10.18710/USXCRS/VI3SJW 10.18710/USXCRS/MCWNRV 10.18710/USXCRS/MZCHPQ 3738 77048 386890 26579 65017 4001 11602 1770 330002 10573 16908 3964 13528 text/plain text/plain text/plain application/vnd.openxmlformats-officedocument.spreadsheetml.sheet application/vnd.openxmlformats-officedocument.spreadsheetml.sheet text/tab-separated-values application/vnd.openxmlformats-officedocument.spreadsheetml.sheet text/plain application/vnd.openxmlformats-officedocument.spreadsheetml.sheet application/vnd.openxmlformats-officedocument.spreadsheetml.sheet text/plain text/plain text/plain 1.1 Creative Commons CC0 1.0 Universal Public Domain Dedication. This dataset is about analyses of two Merkel cell polyomavirus (MCPyV)-negative and two MCPyV–positive Merkel cell lines secretomic proteins. </p>Abstract: Extracellular vesicles or exosomes constitute an evolutionarily conserved mechanism of intercellular signaling. Exosomes are gaining an increasing amount of attention due to their role in pathologies, including malignancy, their importance as prognostic and diagnostic markers, and their potential as a therapeutic tool. Merkel cell carcinoma (MCC) is an aggressive form of skin cancer with a poor prognosis. Because an effective systemic treatment for this cancer type is currently not available, an exosome-based therapy was proposed. However, comprehensive secretome profiling has not been performed for MCC. To help unveil the putative contribution of exosomes in MCC, we studied the protein content of MCC-derived exosomes. Since approximately 80% of all MCC cases contain Merkel cell polyomavirus (MCPyV), the secretomes of two MCPyV-negative and two MCPyV-positive MCC cell lines were compared. We identified with high confidence 164 exosome-derived proteins common for all four cell lines that were annotated in ExoCarta and Vesiclepedia databases. These include proteins implicated in motility, metastasis and tumor progression, such as integrins and tetraspanins, intracellular signaling molecules, chaperones, proteasomal proteins, and translation factors. Additional virus-negative and virus-positiveMCC cell lines should be examined to identify highly representative exosomal proteins that may provide reliable prognostic and diagnostic biomarkers, as well as targets for treatment in the future. Data are available via ProteomeXchange with identifier PXD004198. Odd Fellow Medisinsk-Vitenskapelig Forskningsfond 2A65216