Supporting Data for: Multifocus microscopy with optical sectioning and extended axial resolutionhttps://doi.org/10.18710/J0QX3EStröhl, FlorianHansen, Daniel HenryGrifo, Mireia NagerBirgisdottir, Åsa BirnaDataverseNO2022-09-232023-09-28T22:05:05ZMultifocus microscopy enables recording of entire volumes in a single camera exposure. In dense samples, multifocus microscopy is severely hampered by background haze. We developed a scalable multifocus method that incorporates optical sectioning and offers improved axial resolution capabilities. In our method, dubbed SOLIS, a dithered oblique light-sheet scans the sample volume during a single exposure, while fluorescence from each illuminated plane in the sample is mapped onto a line on the camera with a multifocus optical element. A synchronized rolling shutter readout realizes optical sectioning. This data set contains example images, volumetric stacks, and timelapse videos that demonstrate the technique on endothelial cells (AF488-phalloidin labelled actin) and uncleared engineered human heart tissue (AF488-TOM20 labelled mitochondria).Medicine, Health and Life SciencesPhysicsmulti-focus microscopyfluoroscence microscopy3D microscopylight-sheet microscopyoptical sectioningEnglishStröhl F, Hansen DH, Nàger M, Birgisdottir ÅB. Multifocus microscopy with optical sectioning and high axial resolution. Optica. 2022 9(10), doi, 10.1364/OPTICA.468583, https://doi.org/10.1364/OPTICA.4685832022-09-23Ströhl, FlorianStröhl, FlorianHansen, Daniel H.Grifo, Mireia N.Birgisdottir, Åsa B.2022-08-182022-02-142022-03-17experimental dataCC0 1.0