Replication Data for: Potential for flexible lactate shuttling between astrocytes and neurons to mitigate against diving-induced hypoxiadoi:10.18710/FRCOQZDataverseNO2025-06-131Chiara Ciccone; Sari Elena Dötterer; Sigrid Vold Jensen; Cornelia Geßner; Alexander C. West; Shona H. Wood; David G. Hazlerigg; Lars P. Folkow, 2025, "Replication Data for: Potential for flexible lactate shuttling between astrocytes and neurons to mitigate against diving-induced hypoxia", https://doi.org/10.18710/FRCOQZ, DataverseNO, V1Replication Data for: Potential for flexible lactate shuttling between astrocytes and neurons to mitigate against diving-induced hypoxiadoi:10.18710/FRCOQZChiara CicconeSari Elena DöttererSigrid Vold JensenCornelia GeßnerAlexander C. WestShona H. WoodDavid G. HazleriggLars P. FolkowChiara CicconeSari Elena DöttererSigrid Vold JensenUiT The Arctic University of NorwayTFS2016SWIS3_17_SWIS3_17_SWDataverseNOUiT The Arctic University of NorwayChiara CicconeLars P. FolkowCiccone, Chiara2025-04-07Medicine, Health and Life Sciencesdiving mammalsmitochondrial respirationhooded sealhypoxianeuronastrocytelactate shuttlingThe lack of oxygen, i.e. hypoxia, is a challenging condition for several species. The mammalian brain is particularly sensitive to hypoxia as neurons, the major cerebral cell type, are highly dependent on mitochondrial aerobic metabolism to produce energy and perform their functions. Neurons can preserve their aerobic metabolism by being metabolically coupled with the astrocytes. These are the major type of glia cell in the brain and are anaerobic cells, meaning that they do not need oxygen to produce energy. The end product of the anaerobic metabolism is lactate which, according to the astrocyte-neuron lactate shuttle (ANLS) hypothesis, it is transported to the neurons where it is used as fuel for aerobic metabolism. Recent evidence has shown that this mechanism might not be common to all mammalian brains, particularly for some hypoxia-tolerant species like pinnipeds. Studies on the deep-diving hooded seal (Cystophora cristata) suggest that the shuttling of lactate between cell types in their brain might be reversed, implying a lower dependency on oxygen by neurons (reverse- ANLS). In this dataset we report results from mitochondrial oxygen consumption measurements, quantification of mitochondrial density and size and distribution of the lactate transporter (monocarboxylate transporter 4 – MCT4) in both astrocytes and neurons in the hooded seal brain.2022-05-312024-06-30Experimental data<a href="http://creativecommons.org/publicdomain/zero/1.0">CC0 1.0</a>Ciccone C, Dötterer SE, Vold Jensen S, Geßner C, West AC, Wood SH, Hazlerigg DG and Folkow LP (2025) Potential for flexible lactate shuttling between astrocytes and neurons to mitigate against diving-induced hypoxia. Front. Neuroanat. 19:1607396. https://doi.org/10.3389/fnana.2025.1607396doi.org/10.3389/fnana.2025.1607396Ciccone C, Dötterer SE, Vold Jensen S, Geßner C, West AC, Wood SH, Hazlerigg DG and Folkow LP (2025) Potential for flexible lactate shuttling between astrocytes and neurons to mitigate against diving-induced hypoxia. Front. Neuroanat. 19:1607396. https://doi.org/10.3389/fnana.2025.160739600_README_filetxt.txttext/plainastro_vs_neuro.csvtext/comma-separated-valuesastro_vs_neuro_ratios.csvtext/comma-separated-valuesmct4_fractional_area.csvtext/comma-separated-valuesMFI_count_adj.csvtext/comma-separated-valuesMito_size.csvtext/comma-separated-values