Replication Data for: Enzymatic hydrolysis during in vitro digestion of triacylglycerols in a model system and milk: Isomerisation of diacylglycerols and monoacylglycerolsdoi:10.18710/JPIAZDDataverseNO2026-03-171Eirin Stork, 2026, "Replication Data for: Enzymatic hydrolysis during in vitro digestion of triacylglycerols in a model system and milk: Isomerisation of diacylglycerols and monoacylglycerols", https://doi.org/10.18710/JPIAZD, DataverseNO, V1Replication Data for: Enzymatic hydrolysis during in vitro digestion of triacylglycerols in a model system and milk: Isomerisation of diacylglycerols and monoacylglycerolsdoi:10.18710/JPIAZDEirin StorkØstbø, GardFlytkjær, AndersSkeie, SivJonsson, EinarDevold, ToveEkeberg, DagDevle, Hanne M.Norwegian University of Life SciencesNMBU281207281207DataverseNONorwegian University of Life Sciences (NMBU)Eirin StorkStork, Eirin2026-10-03ChemistryIsomerisationHydrolysisAcylglycerolIn vitro digestionMilk lipidFatty acidThis dataset is from a study where we investigated the enzymatic hydrolysis and degree of isomerisation of diacylglycerols (DAG) and monoacylglycerols (MAG) during in vitro digestion of a triacylglycerol standard (TAG-C14:0/C18:1n-9cis9/C14:0) and bovine milk. The data provides information on the fatty acid (FA) profile of several lipid fractions: triacylglycerols (TAG), DAGs, MAGs, free fatty acids (FFA) and polar lipids (PL), isolated after the in vitro digestion of the TAG standard and commercial whole milk. Data on the lipid profile after gastric digestion of whole milk is also included. In addition, the degree of isomerisation of a sn-2 MAG standard at room temperature and at -20 degrees celcius, during solid phase extraction using amino propyl columns was studied. The data includes chromatograms from the GC_MS analysis of the MAG-samples. Moreover, a direct infusion electrospray ionisation (ESI)-MS/MS method was used to study the difference in the collision induced dissociation (CID) mass spectra between sn-1/sn-3 MAGs and sn-2 MAGs, for the purpose of analysing different MAG isomers. The data includes CID mass spectra for several different standards of 1-MAG and 2-MAG.2020-04-012022-03-01NorwayAkershusAasExperimental data<a href="http://creativecommons.org/publicdomain/zero/1.0">CC0 1.0</a>Stork, E., Skeie, S., Devold, T., Østbø, G., Flytkjær, A., Jonsson, E., Devle, H., & Ekeberg, D. (2025). Enzymatic hydrolysis during in vitro digestion of triacylglycerols in a model system and milk: Isomerisation of diacylglycerols and monoacylglycerols. Food Chemistry Advances, 8, 101041.10.1016/j.focha.2025.101041Stork, E., Skeie, S., Devold, T., Østbø, G., Flytkjær, A., Jonsson, E., Devle, H., & Ekeberg, D. (2025). Enzymatic hydrolysis during in vitro digestion of triacylglycerols in a model system and milk: Isomerisation of diacylglycerols and monoacylglycerols. 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