Replication Data for: Secretomic analysis of extracellular vesicles originating from polyomavirus-negative and polyomavirus-positive Merkel cell carcinoma cell linesdoi:10.18710/USXCRSDataverseNO2019-05-061Konstantinell, Aelita Gloria Virginia, 2019, "Replication Data for: Secretomic analysis of extracellular vesicles originating from polyomavirus-negative and polyomavirus-positive Merkel cell carcinoma cell lines", https://doi.org/10.18710/USXCRS, DataverseNO, V1, UNF:6:xGarNwkr+cO6GOAu/OngFQ== [fileUNF]Replication Data for: Secretomic analysis of extracellular vesicles originating from polyomavirus-negative and polyomavirus-positive Merkel cell carcinoma cell linesdoi:10.18710/USXCRSKonstantinell, Aelita Gloria VirginiaUiT The Arctic University of Norway2A65216DataverseNOUiT The Arctic University of NorwayKonstantinell, Aelita Gloria VirginiaKonstantinell, Aelita Gloria Virginia2019-04-01Medicine, Health and Life SciencesMerkel cell carcinomaMerkel cell polyomavirusExosomeProteomicsThis dataset is about analyses of two Merkel cell polyomavirus (MCPyV)-negative and two MCPyV–positive Merkel cell lines secretomic proteins.</p>Abstract: Extracellular vesicles or exosomes constitute an evolutionarily conserved mechanism of intercellular signaling. Exosomes are gaining an increasing amount of attention due to their role in pathologies, including malignancy, their importance as prognostic and diagnostic markers, and their potential as a therapeutic tool. Merkel cell carcinoma (MCC) is an aggressive form of skin cancer with a poor prognosis. Because an effective systemic treatment for this cancer type is currently not available, an exosome-based therapy was proposed. However, comprehensive secretome profiling has not been performed for MCC. To help unveil the putative contribution of exosomes in MCC, we studied the protein content of MCC-derived exosomes. Since approximately 80% of all MCC cases contain Merkel cell polyomavirus (MCPyV), the secretomes of two MCPyV-negative and two MCPyV-positive MCC cell lines were compared. We identified with high confidence 164 exosome-derived proteins common for all four cell lines that were annotated in ExoCarta and Vesiclepedia databases. These include proteins implicated in motility, metastasis and tumor progression, such as integrins and tetraspanins, intracellular signaling molecules, chaperones, proteasomal proteins, and translation factors. Additional virus-negative and virus-positiveMCC cell lines should be examined to identify highly representative exosomal proteins that may provide reliable prognostic and diagnostic biomarkers, as well as targets for treatment in the future. Data are available via ProteomeXchange with identifier PXD004198.2015-08-012015-12-31Experimental data<a href="http://creativecommons.org/publicdomain/zero/1.0">CC0 1.0</a>Biomarkers Discovery: The Benefit of the Study Exosomes Originated from Merkel Cell Carcinoma Cell Lines. doi: 10.18710/KCW912A. Konstantinell, et al. Secretomic analysis of extracellular vesicles originating from polyomavirus-negative and polyomavirus-positive Merkel cell carcinoma cell lines. Proteomics 2016, 16, 2587-2591.10.1002/pmic.201600223A. Konstantinell, et al. Secretomic analysis of extracellular vesicles originating from polyomavirus-negative and polyomavirus-positive Merkel cell carcinoma cell lines. Proteomics 2016, 16, 2587-2591.Supporting_Information_Table 2.tab388text/tab-separated-valuesUNF:6:xGarNwkr+cO6GOAu/OngFQ==Table2.These37exosomalmarkersoftop100(www.exocarta.og)presentintwopolyomavirus-negativeandtwopolyomavirus-positiveMerkelcellcarcinomacelllines.UNF:6:ARZyFm5kviD7nZuEQ8Px8g==B0.0NaNNaNNaN.NaNNaN38.0UNF:6:gCu/EWfpfjNrx+HRV0Rm2w==CUNF:6:x2Utu3/K0Dh9apme+hwNAQ==DUNF:6:Rov6t2nr0qgw950wD+0wCA==EUNF:6:8KCl6ug3C1Fk1k8YCNq8MQ==FUNF:6:d12WfjF8F3QqD3YmJiFUIg==GUNF:6:85T2DvVLR1Vflq0d0H5B8g==HUNF:6:eS2/3PNRlCxjlPflsjLxjw==01_ReadMe.txtAn overview and short description of the files contained in dataset, which icludes the contact information, what the dataset is about, column headings in tabular data, some abbreviations, and where to find which data = overview of files.text/plainSupporting_Information_Table 1.txttext/plainSupporting_Information_Table 1.xlsxapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetSupporting_Information_Table 2.txttext/plainSupporting_Information_Table 3.txttext/plainSupporting_Information_Table 3.xlsxapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetSupporting_Information_Table 4.txttext/plainSupporting_Information_Table 4.xlsxapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetSupporting_Information_Table 5.txttext/plainSupporting_Information_Table 5.xlsxapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetSupporting_Information_Table 6.txttext/plainSupporting_Information_Table 6.xlsxapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheet