An RNA-seq time series of the medaka pituitary gland during sexual maturation (doi:10.18710/HTCXRN)

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An RNA-seq time series of the medaka pituitary gland during sexual maturation

doi:10.18710/HTCXRN

DataverseNO

2022-09-23

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Ager-Wick, Eirill; Maugars, Gersende; Von Krogh, Kristine; Weltzien, Finn-Arne; Henkel, Christiaan, 2022, "An RNA-seq time series of the medaka pituitary gland during sexual maturation", https://doi.org/10.18710/HTCXRN, DataverseNO, V1

An RNA-seq time series of the medaka pituitary gland during sexual maturation

doi:10.18710/HTCXRN

Ager-Wick, Eirill (Norwegian University of Life Sciences (NMBU))

Maugars, Gersende (Norwegian University of Life Sciences (NMBU))

Von Krogh, Kristine (Norwegian University of Life Sciences (NMBU))

Weltzien, Finn-Arne (Norwegian University of Life Sciences (NMBU))

Henkel, Christiaan (Norwegian University of Life Sciences (NMBU))

Ager-Wick, Eirill

Maugars, Gersende

Von Krogh, Kristine

Weltzien, Finn-Arne

Henkel, Christiaan

Norwegian University of Life Sciences (NMBU)

STAR

Samtools

htseq-count

R

edgeR

cqn

251307

255601

248828

DataverseNO

Norwegian University of Life Sciences (NMBU)

Henkel, Christiaan

Henkel, Christiaan

2022-09-05

https://doi.org/10.18710/HTCXRN

Agricultural Sciences, Medicine, Health and Life Sciences, Pituitary gland, Teleost fish, Transcriptomics, Medaka, Histology

Directing both organismal homeostasis and physiological adaptation, the pituitary is a key endocrine gland in all vertebrates. One of its major tasks is to coordinate sexual maturation though the production and release of hormones stimulating gonad development. In order to study its developmental dynamics in the model fish medaka (Oryzias latipes), we sampled both the pituitary and the ovaries of 68 fish across the entire course of sexual maturation. We subsequently analyzed the pituitary glands using RNA-seq optimized for low input, revealing the time courses of production for individual peptide hormones.

2018-2018

Experimental data

Histology imaging

Transcriptomics

<a href="http://creativecommons.org/publicdomain/zero/1.0">CC0 1.0</a>

Raw Illumina sequencing data for this dataset are available at the NCBI Gene Expression Omnibus, accession GSE179598 (https://identifiers.org/geo:GSE179598)

Ager-Wick, E., Maugars, G., von Krogh, K. et al. An RNA-seq time series of the medaka pituitary gland during sexual maturation. Sci Data 10, 62 (2023).

10.1038/s41597-023-01967-w

Ager-Wick, E., Maugars, G., von Krogh, K. et al. An RNA-seq time series of the medaka pituitary gland during sexual maturation. Sci Data 10, 62 (2023).

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00_readme.txt

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Description of the files

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01_medaka_R_processing_2022.txt

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R code used in data processing

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02_medaka_samples_2022.txt

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Information about each sampled fish

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03_medaka_counts_2022.txt

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RNA-seq count results, per sample and per gene

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04_medaka_scaled_2022.txt

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Top genes processed using scaling normalization

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05_medaka_quantile_2022.txt

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Top genes processed using quantile normalization

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06_medaka1_B_3_40x.png

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Ovaries histology image for fish 1

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Ovaries histology image for fish 2

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Ovaries histology image for fish 4

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Ovaries histology image for fish 5

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Ovaries histology image for fish 6

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Ovaries histology image for fish 10

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Ovaries histology image for fish 14

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Ovaries histology image for fish 18

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Ovaries histology image for fish 19

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Ovaries histology image for fish 22

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Ovaries histology image for fish 24

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Ovaries histology image for fish 26

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Ovaries histology image for fish 28

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Ovaries histology image for fish 29

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Ovaries histology image for fish 32

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Ovaries histology image for fish 33

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Ovaries histology image for fish 41

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Ovaries histology image for fish 47

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Ovaries histology image for fish 52

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Ovaries histology image for fish 54

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Ovaries histology image for fish 55

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Ovaries histology image for fish 60

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Ovaries histology image for fish 63

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Ovaries histology image for fish 64

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Ovaries histology image for fish 67

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Ovaries histology image for fish 68

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Ovaries histology image for fish 71

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Ovaries histology image for fish 75

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Ovaries histology image for fish 78

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Ovaries histology image for fish 82

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Ovaries histology image for fish 88

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Ovaries histology image for fish 89

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Ovaries histology image for fish 94

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Ovaries histology image for fish 97

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Ovaries histology image for fish 98

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Ovaries histology image for fish 99

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Ovaries histology image for fish 100

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Ovaries histology image for fish 103

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Ovaries histology image for fish 104

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Ovaries histology image for fish 106

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Ovaries histology image for fish 108

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Ovaries histology image for fish 109

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Ovaries histology image for fish 110

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Ovaries histology image for fish 111

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Ovaries histology image for fish 115

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Ovaries histology image for fish 117

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