Replication Data for: Elevated methane alters dissolved organic matter composition in the Arctic Ocean cold seeps (doi:10.18710/U3YMFQ)

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Replication Data for: Elevated methane alters dissolved organic matter composition in the Arctic Ocean cold seeps

doi:10.18710/U3YMFQ

DataverseNO

2023-09-01

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Sert, Muhammed Fatih; Kekäläinen, Timo; Ferré, Bénédicte; de Groot, Tim R., 2023, "Replication Data for: Elevated methane alters dissolved organic matter composition in the Arctic Ocean cold seeps", https://doi.org/10.18710/U3YMFQ, DataverseNO, V1

Replication Data for: Elevated methane alters dissolved organic matter composition in the Arctic Ocean cold seeps

doi:10.18710/U3YMFQ

Sert, Muhammed Fatih (UiT The Arctic University of Norway)

Kekäläinen, Timo (Department of Chemistry, University of Eastern Finland)

Ferré, Bénédicte (UiT The Arctic University of Norway)

de Groot, Tim R. (UiT The Arctic University of Norway)

Centre for Arctic Gas Hydrate, Environment and Climate (CAGE), UiT-The Arctic University of Norway

Department of Geosciences, UiT The Arctic University of Norway

Department of Chemistry, University of Eastern Finland

Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Institute for Sea Research

Silyakova, Anna

Niemann, Helge

Gründger, Friederike

Jänis, Janne

Kalenitchenko, Dimitri

UiT The Arctic University of Norway

R

R Studio

project number 223259

project number 320100

EU FT-ICR MS project; grant agreement 731077

DataverseNO

UiT The Arctic University of Norway

Sert, Muhammed Fatih

Sert, Muhammed Fatih

2023-08-24

https://doi.org/10.18710/U3YMFQ

Earth and Environmental Sciences, Dissolved organic matter, methane, cold seeps, Fourier-transform ion cyclotron resonance mass spectrometry

<p>This dataset contains water column measurements, dissolved organic matter compositions, and hydrographical profiles collected from the Norskebanken seep site at Northern Svalbard, Arctic Ocean. Data are presented along with the R codes for the replication of analyses, and figures that were added in our submitted paper. </p> <p>We recommend using R (v4.3.1) and R Studio (2023.06.1) to run R project file (.Rproj) which can access all codes and data. Running codes in the given order will create corresponding data tables and all the figures that were used in the article.</p>

<p>Cold seeps release methane (CH4) from the seafloor to the water column, which fuels microbially mediated aerobic methane oxidation (MOx). Methane oxidising bacteria (MOB) utilise excess methane, and the MOB biomass serves as a carbon source in the food web. Yet, it remains unclear if and how MOx modifies the composition of dissolved organic matter (DOM) in cold seeps. We investigated MOx rates, DOM compositions and the microbial community during ex-situ incubations of seawater collected from an active cold seep site at Norskebanken (north of the Svalbard archipelago) in the Arctic Ocean. For comparison, we used seawater collected from a control site (a non-seep environment). Samples were incubated with and without methane amendments. Samples amended with methane (~1 µM final concentration) showed elevated rates of MOx in both seep and non-seep incubations. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) analyses showed that the number of DOM formulas (i.e., molecular diversity) increased by up to 39 % in these incubations. In contrast, the number of formulas decreased by 20 % in samples not amended with methane, both from non-seep and seep locations. DOM composition was thus altered towards a more diverse and heterogeneous composition along with elevated methanotrophic activity in methane-amended conditions. In addition to microbial DOM production, abating microbial diversity indicates that elevated DOM diversity was potentially related to grazing pressure on bacteria. The diversity of DOM constituents, therefore, likely increased with the variety of decaying cells contributing to DOM production. Furthermore, based on a principal coordinate analysis, we show that the final DOM composition of non-seep samples amended with methane became more resemblant to that of seep samples. This suggests that methane intrusions will affect water column DOM dynamics similarly, irrespective of the water column’s methane history. </p>

2020-11-02-2020-11-16

2020-11-02-2020-11-16

Svalbard and Jan Mayen

Norskebanken

• West Bounding Longitude: 14.00
• East Bounding Longitude: 14.30
• South Bounding Latitude: 80.30
• North Bounding Latitude: 80.36

Survey data

Experimental data

<p>The files 0_incubation_dataset.Rproj, 1_ctd.R, 2_env_data.R, 3_DOM_plots.Rmd and 4_DOM_pcoa.Rmd are licensed under:</p> <p>MIT License</p> <p>Copyright (c) 2023 Muhammed Fatih Sert</p> <p>Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:</p> <p>The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software.</p> <p>THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.</p> <p>---</p> <p>The files 00_readme.txt, ada_neg.RData, ada_pos.RData, env_data.csv and Sta0205.cnv are licensed under CC0 1.0 Universal (CC0 1.0) Public Domain Dedication: <r> https://creativecommons.org/publicdomain/zero/1.0/ </r> </p> <p>---</p> <p>Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation shown on the dataset page. </p>

https://github.com/fatihserta/Incubation_2022

Muhammed Fatih Sert, Hannah D. Schweitzer, Tim R. de Groot, Timo Kekäläinen, Janne Jänis, Hans C. Bernstein, Bénédicte Ferré, Friederike Gründger, Dimitri Kalenitchenko, and Helge Niemann. Elevated methane alters dissolved organic matter composition in the Arctic Ocean cold seeps. Submitted.

Muhammed Fatih Sert, Hannah D. Schweitzer, Tim R. de Groot, Timo Kekäläinen, Janne Jänis, Hans C. Bernstein, Bénédicte Ferré, Friederike Gründger, Dimitri Kalenitchenko, and Helge Niemann. Elevated methane alters dissolved organic matter composition in the Arctic Ocean cold seeps. Submitted.

Ferré, B., Moser, M., Dølven, K. O., Sert, F., Stetzler, M., Savini, A., et al. (2020). CAGE 20-7 Cruise report: Sediment and water column analyses around flares at Norskebanken, Hinlopen and offshore Prins Karls Forland.

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00_readme.txt

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0_INCUBATION_dataset.Rproj

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1_ctd.R

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2_env_data.R

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3_DOM_plots.Rmd

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4_DOM_pcoa.Rmd

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adata_neg.RData

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adata_pos.RData

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Sta0211.cnv

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