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Part 1: Document Description
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Citation |
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Title: |
Supplementary Data for: Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis |
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Identification Number: |
doi:10.18710/XZB5KI |
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Distributor: |
DataverseNO |
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Date of Distribution: |
2023-05-09 |
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Version: |
1 |
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Bibliographic Citation: |
Rainsford, Philip; Rylandsholm, Fredrik G.; Jakubec, Martin; Silk, Mitchell; Juskewitz, Eric; Ericson, Johanna U.; Svendsen, John S.; Engh, Richard A.; Isaksson, Johan, 2023, "Supplementary Data for: Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis", https://doi.org/10.18710/XZB5KI, DataverseNO, V1 |
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Citation |
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Title: |
Supplementary Data for: Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis |
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Identification Number: |
doi:10.18710/XZB5KI |
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Authoring Entity: |
Rainsford, Philip (UiT The Arctic University of Norway) |
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Rylandsholm, Fredrik G. (UiT The Arctic University of Norway) |
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Jakubec, Martin (UiT The Arctic University of Norway) |
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Silk, Mitchell (UiT The Arctic University of Norway) |
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Juskewitz, Eric (UiT The Arctic University of Norway) |
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Ericson, Johanna U. (UiT The Arctic University of Norway) |
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Svendsen, John S. (UiT The Arctic University of Norway) |
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Engh, Richard A. (UiT The Arctic University of Norway) |
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Isaksson, Johan (UiT The Arctic University of Norway) |
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Producer: |
UiT The Arctic University of Norway |
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Date of Production: |
2022-10-01 |
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Software used in Production: |
Biacore T200 Control and Evaluation Software |
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Software used in Production: |
MO.Control |
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Grant Number: |
DigiBiotics project - ID 269425 |
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Grant Number: |
AntiBioSpec project - Cristin ID 20161326 |
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Grant Number: |
NanoAMP project - ID 275186 |
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Distributor: |
DataverseNO |
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Distributor: |
UiT The Arctic University of Norway |
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Access Authority: |
Jakubec, Martin |
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Access Authority: |
Rainsford, Philip |
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Depositor: |
Jakubec, Martin |
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Date of Deposit: |
2023-05-04 |
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Holdings Information: |
https://doi.org/10.18710/XZB5KI |
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Study Scope |
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Keywords: |
Chemistry, Medicine, Health and Life Sciences, SPR, Surface Plasmon Resonance, Microscale Thermophoresis, MST, Lipid nanodiscs, antimicrobials, Lipid vesicles, binding affinity |
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Abstract: |
This dataset contains raw data traces from Surface Plasmon Resonance (SPR) and Microscale Thermophoresis (MST) experiments aimed at determining the binding affinity of peptides with lipid vesicles and lipid nanodiscs. The SPR data consists of responses obtained from cycles of peptide injection over the lipid membrane, with varying peptide concentrations. The MST data includes fluorescence responses obtained from capillaries with different ratios of tested peptides and lipids. Tested hexapeptides were 1-c(WWWRRR), 2-c(WRWRWR), 3-c(WWWKKK), 4-c(WKWKWK) and 5-c(LWwNKr). The lipid compositions used in the experiments were pure 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), DMPC with 5% (w/w) 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DMPG), or DMPC with 10% (w/w) lipopolysaccharides from Escherichia coli O111:B4. These data traces are essential for measuring KP (lipid partitioning constant), understanding the peptide-lipid interaction and identifying potential candidates for further analysis. |
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Date of Collection: |
2021-09-01-2022-09-30 |
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Kind of Data: |
experimental data |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Notes: |
<a href="http://creativecommons.org/publicdomain/zero/1.0">CC0 1.0</a> |
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Other Study Description Materials |
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Related Materials |
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Matlab scripts used for processing SPR files: https://github.com/MarJakubec/SPR |
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Related Publications |
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Citation |
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Title: |
Rainsford P, Rylandsholm F, Jakubec M, Juskewitz E, Silk M, Engh RA, et al. Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis. ChemRxiv. Cambridge: Cambridge Open Engage; 2022 |
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Identification Number: |
10.26434/chemrxiv-2022-hpf6f-v2 |
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Bibliographic Citation: |
Rainsford P, Rylandsholm F, Jakubec M, Juskewitz E, Silk M, Engh RA, et al. Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis. ChemRxiv. Cambridge: Cambridge Open Engage; 2022 |
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00_README.txt |
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text/plain |
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MST_SMA-QA_DMPC_fluor.txt |
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text/plain |
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MST_SMA-QA_DMPC_t-jump.txt |
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text/plain |
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MST_SMA-QA_DMPG_fluor.txt |
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text/plain |
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MST_SMA-QA_DMPG_t-jump.txt |
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text/plain |
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MST_SMA-QA_fluor.txt |
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text/plain |
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MST_SMA-QA_t-jump.txt |
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text/plain |
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MST_SMA_DMPC_fluor.txt |
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text/plain |
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MST_SMA_DMPC_t-jump.txt |
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text/plain |
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MST_SMA_DMPG_fluor.txt |
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text/plain |
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MST_SMA_DMPG_t-jump.txt |
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text/plain |
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MST_SMA_fluor.txt |
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text/plain |
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MST_SMA_t-jump.txt |
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text/plain |
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MST_Ves_DMPC_fluor.txt |
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text/plain |
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MST_Ves_DMPC_t-jump.txt |
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text/plain |
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MST_Ves_DMPG_fluor.txt |
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text/plain |
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MST_Ves_DMPG_t-jump.txt |
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text/plain |
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SPR_c(LWwNKr)_DMPC_Channel1.txt |
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SPR_c(LWwNKr)_DMPC_Channel2.txt |
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SPR_c(LWwNKr)_DMPC_Channel3.txt |
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SPR_c(LWwNKr)_DMPC_Channel4.txt |
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SPR_c(LWwNKr)_DMPC_PG_Channel1.txt |
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SPR_c(LWwNKr)_DMPC_PG_Channel2.txt |
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SPR_c(LWwNKr)_DMPC_PG_Channel3.txt |
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SPR_c(LWwNKr)_DMPC_PG_Channel4.txt |
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SPR_c(WKWKWK)_DMPC_Channel1.txt |
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SPR_c(WKWKWK)_DMPC_Channel2.txt |
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text/plain |
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SPR_c(WKWKWK)_DMPC_Channel3.txt |
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SPR_c(WKWKWK)_DMPC_Channel4.txt |
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SPR_c(WKWKWK)_DMPC_PG_Channel1.txt |
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SPR_c(WKWKWK)_DMPC_PG_Channel2.txt |
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SPR_c(WKWKWK)_DMPC_PG_Channel3.txt |
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SPR_c(WKWKWK)_DMPC_PG_Channel4.txt |
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SPR_c(WRWRWR)_DMPC_Channel1.txt |
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SPR_c(WRWRWR)_DMPC_Channel2.txt |
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SPR_c(WRWRWR)_DMPC_Channel3.txt |
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SPR_c(WRWRWR)_DMPC_Channel4.txt |
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SPR_c(WRWRWR)_DMPC_PG_Channel1.txt |
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SPR_c(WRWRWR)_DMPC_PG_Channel2.txt |
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SPR_c(WRWRWR)_DMPC_PG_Channel3.txt |
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SPR_c(WRWRWR)_DMPC_PG_Channel4.txt |
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SPR_c(WWWKKK)_DMPC_Channel1.txt |
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SPR_c(WWWKKK)_DMPC_Channel2.txt |
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SPR_c(WWWKKK)_DMPC_Channel3.txt |
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SPR_c(WWWKKK)_DMPC_Channel4.txt |
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SPR_c(WWWKKK)_DMPC_PG_Channel1.txt |
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SPR_c(WWWKKK)_DMPC_PG_Channel2.txt |
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SPR_c(WWWKKK)_DMPC_PG_Channel3.txt |
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SPR_c(WWWKKK)_DMPC_PG_Channel4.txt |
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SPR_c(WWWRRR)_DMPC_Channel1.txt |
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SPR_c(WWWRRR)_DMPC_Channel2.txt |
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SPR_c(WWWRRR)_DMPC_Channel3.txt |
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SPR_c(WWWRRR)_DMPC_Channel4.txt |
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SPR_c(WWWRRR)_DMPC_PG_Channel1.txt |
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SPR_c(WWWRRR)_DMPC_PG_Channel2.txt |
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SPR_c(WWWRRR)_DMPC_PG_Channel3.txt |
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SPR_c(WWWRRR)_DMPC_PG_Channel4.txt |
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text/plain |