10.18710/IIBITUNur Cahyo, HendraHendraNur Cahyo0000-0001-8773-7440Norwegian University of Life Sciences (NMBU)DataverseNO2026Agricultural SciencesMethaneimmune responsefeed efficiencyrumen microbesSchwarm, AngelaAngelaSchwarmNorwegian University of Life Sciences (NMBU)Norwegian University of Life Sciences (NMBU)Niu, PuchunPuchunNiuPope, Phillip BPhillip BPopeGimsa, UlrikeUlrikeGimsaKuhla, BjoernBjoernKuhlaSchwarm, AngelaAngelaSchwarmNorwegian University of Life Sciences (NMBU)20202025-11-132026-02-062020-01-01/2020-12-30Experimental data10.3168/jds.2025-2692516126313941260472957text/plaintext/comma-separated-valuestext/comma-separated-valuestext/comma-separated-valuesapplication/pdf1.0CC BY 4.0The supplementary material provides information for the article published in Journal of Dairy Science, entitled "Methane category, immune response, feed efficiency, and rumen microbial community in lactating dairy cows". This study aims to assess relationships of enteric methane (CH4) yield (g/kg of dry matter intake (DMI)) with immune response, feed efficiency (energy corrected milk (ECM)/DMI), and rumen microbiome in dairy cows, both in early and in late lactation. The DMI, body weight, ECM yield, and CH4 emission were measured in respiration chambers in early (n = 20, 32 ± 7 days in milk (DIM)) and non-pregnant late lactating (n=14, 359 ± 90 DIM) multiparous Holstein cows. The in vitro immune response was studied in response to (1) lipopolysaccharide using whole blood, and (2) phytohemagglutinin and concanavalin A using peripheral blood mononuclear cells. The DNA was extracted from rumen content samples (esophageal tubing) for 16S rRNA microbial analysis. Cows were divided retrospectively into an equal number of cows with low (LMY) and high (HMY) CH4 yield within each lactation group.R, 4.4.1Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, GermanyEU Horizon (SmarCow project - Transnational Access)730925Research Council of Norway (ViableCow)316157Novo Nordisk Foundation (SuPaCow)0054575Australian Research Council (Future Fellowship)FT230100560