10.18710/JEN4SBOpstad, Ida S.Ida S.Opstad0000-0003-4462-4600UiT The Arctic University of NorwayDataverseNO2021Medicine, Health and Life SciencesPhysicsTIRFwaveguide imagingfluctuation imagingsuper-resolutionactinsalmon keratocytesfluctuation imagingchip microscopyOpstad, Ida S.Ida S.OpstadUiT The Arctic University of NorwayUiT The Arctic University of NorwayHansen, Daniel H.Daniel H.HansenUiT The Arctic University of NorwayUiT The Arctic University of Norway2020-09-042021-03-012023-11-012020-09-04/2020-09-04Experimental10.1364/OE.42380981356993519412text/plainapplication/zip1.4CC0 1.0<p>The chip-based total internal reflection fluorescence microscopy data is of fixed salmon keratocytes labelled using phalloidin-ATTO647N and acquired using 660 nm excitation, 10X 0.3NA water dipping objective.</p> <p></p> <p>Abstract of publication: Photonic chip-based total internal reflection fluorescence microscopy (c-TIRFM) is an emerging technology enabling a large TIRF excitation area decoupled from the detection objective. Additionally, due to the inherent multimodal nature of wide waveguides, it is a convenient platform for introducing temporal fluctuations in the illumination pattern. The fluorescence fluctuation-based nanoscopy technique Multiple Signal Classification Algorithm (MUSICAL) does not assume stochastic independence of the emitter emission and can therefore exploit fluctuations arising from other sources, as such multimodal illumination patterns. In this work, we demonstrate and verify the utilization of fluctuations in the illumination for super-resolution imaging using MUSICAL on actin in salmon keratocytes. The resolution improvement was measured to be 2.2–3.6-fold compared to the corresponding conventional images. DHH built the imaging system and contributed in the data acquisition</p>TromsøUiT The Arctic University of NorwayUiT strategic fundingThe Research Council of Norway301401European Research Council749666European Research Council836355