10.18710/SHSZXSStröhl, FlorianFlorianStröhl0000-0002-2603-0780UiT The Arctic University of NorwayDataverseNO2023EngineeringMedicine, Health and Life SciencesPhysicsmicroscopyultra-violetliver sinusoidal endothelial cellStröhl, FlorianFlorianStröhlUiT The Arctic University of NorwayUiT The Arctic University of NorwayTheThe Arctic University of NorwayUiT The Arctic University of NorwayTheThe Arctic University of NorwayUiT The Arctic University of Norway2023-02-152023-09-282021-06-02/2021-11-18images10.1038/s41377-023-01105-6114841152064731152057628801681152066454755150429124854433257524609421353157601465760146576014657601461982225438341028836119035355364531737191413577621440148500text/plainimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tifftext/x-matlabtext/x-matlabtext/x-matlabtext/x-matlabtext/x-matlabtext/x-matlabtext/x-matlabtext/x-matlabtext/x-matlabtext/x-matlabtext/x-matlabtext/x-matlabtext/x-matlabimage/tifftext/x-matlab1.1CC0 1.0This repository contains underlying image data, which was used for figures in the manuscript and MATLAB code to generate extinction coefficient maps as well as MATLAB code to determine the direction of "half-ring" illumination from differential phase contrast (DPC) raw data.Summary from the manuscript: We presented a C-band UV microscope operating at 275 nm, which provides high-contrast high-resolution label-free microscopy. Through a series of oblique illuminations, we showed differential phase contrast (DPC) illumination in this wavelength regime and achieved a 7- to 300-fold improvement over other methods. The phase retrieval algorithm of quantitative DPC microscopy permitted us to calculate extinction coefficients within liver sinusoidal endothelial cells (LSECs). With a resolution down to 215 nm, we could resolve individual fenestrations within their sieve plates, and demonstrated the quantitative character extinction coefficient imaging. The UVC illumination allowed us to utilize intrinsic fluorescence from proteins and amino acids as an orthogonal imaging modality. We exploited this in performing correlative label-free imaging with both autofluorescence and differential phase contrast.DPC_illumination_finder.m, v1.0DPC_single_image_reconstruction.m, v1.0European Union’s Horizon 2020 Marie Skłodowska-Curie Action836355European Union’s Horizon 2020 Marie Skłodowska-Curie Action766181Publication fund of UiT The Arctic University of Norway