Persistent Identifier
|
doi:10.18710/JEN4SB |
Publication Date
|
2021-05-27 |
Title
| Replication data for: Fluorescence fluctuation-based super-resolution microscopy using multimodal waveguided illumination |
Author
| Opstad, Ida S. (UiT The Arctic University of Norway) - ORCID: 0000-0003-4462-4600 |
Point of Contact
|
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Opstad, Ida S. (UiT The Arctic University of Norway) |
Description
| The chip-based total internal reflection fluorescence microscopy data is of fixed salmon keratocytes labelled using phalloidin-ATTO647N and acquired using 660 nm excitation, 10X 0.3NA water dipping objective.
Abstract of publication: Photonic chip-based total internal reflection fluorescence microscopy (c-TIRFM) is an emerging technology enabling a large TIRF excitation area decoupled from the detection objective. Additionally, due to the inherent multimodal nature of wide waveguides, it is a convenient platform for introducing temporal fluctuations in the illumination pattern. The fluorescence fluctuation-based nanoscopy technique Multiple Signal Classification Algorithm (MUSICAL) does not assume stochastic independence of the emitter emission and can therefore exploit fluctuations arising from other sources, as such multimodal illumination patterns. In this work, we demonstrate and verify the utilization of fluctuations in the illumination for super-resolution imaging using MUSICAL on actin in salmon keratocytes. The resolution improvement was measured to be 2.2–3.6-fold compared to the corresponding conventional images. DHH built the imaging system and contributed in the data acquisition (2021-03-01) |
Subject
| Medicine, Health and Life Sciences; Physics |
Keyword
| TIRF
waveguide imaging
fluctuation imaging
super-resolution
actin
salmon keratocytes
fluctuation imaging
chip microscopy |
Related Publication
| Ida S. Opstad, Daniel H. Hansen, Sebastian Acuña, Florian Ströhl, Anish Priyadarshi, Jean-Claude Tinguely, Firehun T. Dullo, Roy A. Dalmo, Tore Seternes, Balpreet S. Ahluwalia, and Krishna Agarwal, "Fluorescence fluctuation-based super-resolution microscopy using multimodal waveguided illumination," Opt. Express 29, 23368-23380 (2021) doi: 10.1364/OE.423809 https://doi.org/10.1364/OE.423809 |
Language
| English |
Producer
| UiT The Arctic University of Norway (UiT) https://en.uit.no/ |
Production Date
| 2020-09-04 |
Production Location
| Tromsø |
Contributor
| Data Collector : Hansen, Daniel H. |
Funding Information
| UiT The Arctic University of Norway: UiT strategic funding
The Research Council of Norway: 301401
European Research Council: 749666
European Research Council: 836355 |
Distributor
| UiT The Arctic University of Norway (UiT The Arctic University of Norway) (UiT) https://dataverse.no/dataverse/uit |
Depositor
| Opstad, Ida Sundvor |
Deposit Date
| 2021-03-01 |
Date of Collection
| Start Date: 2020-09-04 ; End Date: 2020-09-04 |
Data Type
| Experimental |