Persistent Identifier
|
doi:10.18710/AAJYOW |
Publication Date
|
2021-05-03 |
Title
| The proteome and secretome of rat liver sinusoidal endothelial cells in early primary culture, and effects of dexamethasone |
Author
| Bhandari, Sabin (UiT The Arctic University of Norway) - ORCID: 0000-0003-3837-027X |
Point of Contact
|
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Sørensen, Karen Kristine (UiT The Arctic University of Norway) |
Description
| LSECs from male Sprague Dawley rats were cultured on collagen type-I in 5% oxygen atmosphere in DMEM with serum-free supplements for 2-24h. Quantitative proteomics using a tandem mass tag (TMT)6-plex strategy on Q-Exactive mass spectrometer. The worksheet contains the normalized reporter intensities for the 3 biological replicates for 5 samples representing 2h control, 24h control, 24h+IL-1beta, 24h + Dex, 24h + Dex and IL-1 beta (2021-04-29) |
Subject
| Medicine, Health and Life Sciences |
Keyword
| Rats (MeSH) https://www.ncbi.nlm.nih.gov/mesh/D051381
Liver (MeSH) https://www.ncbi.nlm.nih.gov/mesh/D008099
Proteome (MeSH) https://www.ncbi.nlm.nih.gov/mesh/D020543
Proteomics (MeSH) https://www.ncbi.nlm.nih.gov/mesh/D040901
Dexamethasone (MeSH) https://www.ncbi.nlm.nih.gov/mesh/D003907 |
Related Publication
| Li R, Bhandari S, Martinez-Zubiaurre I, Bruun J-A, Urbarova I, Smedsrød B, et al. (2022) Changes in the proteome and secretome of rat liver sinusoidal endothelial cells during early primary culture and effects of dexamethasone. PLoS ONE 17(9): e0273843. https://doi.org/10.1371/journal.pone.0273843, doi: 10.1371/journal.pone.0273843 https://doi.org/10.1371/journal.pone.0273843 |
Language
| English |
Producer
| UiT The Arctic University of Norway (UiT) https://en.uit.no/ |
Production Date
| 2016-06-01 |
Production Location
| Proteomics and Metabolomics Core Facility (PRiME), UiT The Arctic University of Norway |
Contributor
| Data Curator : Bruun, Jack-Ansgar
Data Curator : Bhandari, Sabin
Hosting Institution : UiT The Arctic University of Norway
Project Leader : Sørensen, Karen
Project Member : Li, Ruomei
Project Member : Martinez, Inigo
Project Member : Urbarova, Ilona
Project Member : Smedsrød, Bård
Project Member : Simon-Santamaria, Jaione
Research Group : Vascular Biology Research Group (VBRG)
Funder : UiT The Arctic University of Norway |
Distributor
| UiT The Arctic University of Norway (UiT The Arctic University of Norway) https://dataverse.no/dataverse/uit |
Depositor
| Bhandari, Sabin |
Deposit Date
| 2021-04-29 |
Date of Collection
| Start Date: 2016-03-01 |
Data Type
| TMT-Sixplex labelled proteomic data |
Related Dataset
| The mass spectrometry proteomics raw data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD029241. Available at: https://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD029241; The whole processed proteome is included in Supporting Information (S1 Dataset) and paper. Available at: https://doi.org/10.1371/journal.pone.0273843 |