Persistent Identifier
|
doi:10.18710/PDCLAS |
Publication Date
|
2021-09-23 |
Title
| 3DSIM data of mitochondria in the cardiomyoblast cell-line H9c2 adapted to either glucose or galactose |
Author
| Opstad, Ida S. (UiT The Arctic University of Norway) - ORCID: 0000-0003-4462-4600 |
Point of Contact
|
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Opstad, Ida S. (UiT The Arctic University of Norway) |
Description
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Series of three-dimensional structured illumination microscopy (3DSIM) of the rat cardiomyoblast cell-line H9c2 adapted to either glucose (GLU) or galactose (GAL). The outer mitochondrial membranes are labeled with the double tag mCherry-EGFP-OMP-25TM (dTag), i.e., both a red and green fluorescent protein variant. dTag can be used as a sensor for acidity (or lysosomal degradation), as the green fluorescence is lost sooner in an acidic environment than the red fluorescence. Where indicated, the samples are additionally labeled with LysoTracker Deep Red (LTDR). The aim of the study was to test the feasibility of multi-color 3DSIM for the examination of mitochondria-derived vesicles (MDVs) in both living and fixed cells. (2022-01-14) |
Subject
| Medicine, Health and Life Sciences |
Keyword
| 3DSIM
SIM
Structured illumination microscopy
super-resolution microscopy
live-cell
fixed-cell
mitochondria
mitochondrial nanotubes
MDVs
mitochondria-derived vesicles
mCherry-EGFP
mitochondrial outer membrane protein 25 transmembrane domain
OMP25-TM
stable expression
galactose adapted
H9c2
cardiomyoblasts
outer mitochondrial membrane
lysosomes
lysotracker deep red |
Related Publication
| Opstad, I. S., Godtliebsen, G., Ahluwalia, B. S., Myrmel, T., Agarwal, K., & Birgisdottir, Å. B. (2022). Mitochondrial dynamics and quantification of mitochondria‐derived vesicles in cardiomyoblasts using structured illumination microscopy. Journal of Biophotonics, 15(2), e202100305. doi: 10.1002/jbio.202100305 https://doi.org/10.1002/jbio.202100305
Opstad, I. S., Godtliebsen, G., Ströhl, F., Myrmel, T., Ahluwalia, B. S., Agarwal, K., & Birgisdottir, Å. B. (2022). Three-dimensional structured illumination microscopy data of mitochondria and lysosomes in cardiomyoblasts under normal and galactose-adapted conditions. Scientific Data, 9(1), 1-7. doi: 10.1038/s41597-022-01207-7 https://doi.org/10.1038/s41597-022-01207-7 |
Language
| English |
Producer
| UiT The Arctic University of Norway (UiT) https://en.uit.no/ |
Production Date
| 2021-06-28 |
Production Location
| UiT |
Contributor
| Project Leader : Birgisdottir, Åsa Birna
Project Leader : Myrmel, Truls
Researcher : Godtliebsen, Gustav
Project Leader : Agarwal, Krishna
Sponsor : Ahluwalia, Balpreet S.
Data Curator : Sarre, Aili
Data Curator : Conzett, Philipp |
Funding Information
| Northern Norway Regional Health Authority: HNF1449-19
UiT The Arctic University of Norway: VirtualStain; project number 2061348 |
Distributor
| UiT The Arctic University of Norway (UiT The Arctic University of Norway) https://dataverse.no/dataverse/uit |
Depositor
| Opstad, Ida Sundvor |
Deposit Date
| 2021-05-30 |
Date of Collection
| Start Date: 2021-04-20 ; End Date: 2021-05-11 |
Data Type
| Experimental |