Persistent Identifier
|
doi:10.18710/5E8VUI |
Publication Date
|
2020-10-13 |
Title
| Replication data for: Following the Fate of Dye-Containing Liposomes in Vitro |
Author
| Cauzzo, Jennifer (UiT The Arctic University of Norway) - ORCID: 0000-0002-4440-5101
Nystad, Mona (UiT The Arctic University of Norway; University Hospital of North Norway) - ORCID: 0000-0002-0555-6951
Holsæter, Ann Mari (UiT The Arctic University of Norway) - ORCID: 0000-0002-3513-5314
Basnet, Purusotam (UiT The Arctic University of Norway; University Hospital of North Norway) - ORCID: 0000-0001-8612-9558
Skalko-Basnet, Natasa (UiT The Arctic University of Norway) - ORCID: 0000-0002-4301-2840 |
Point of Contact
|
Use email button above to contact.
Skalko-Basnet, Natasa (UiT The Arctic University of Norway) |
Description
| This dataset contains the experimental work associated to the publication "Following the Fate of Dye-Containing Liposomes In Vitro" Int. J. Mol. Sci. 2020, 21(14), 4847; https://doi.org/10.3390/ijms21144847.
The rather limited success of translation from basic research to clinical application has been highlighted as a major issue in the nanomedicine field. To identify the factors influencing the applicability of nanosystems as drug carriers and potential nanomedicine, we focused on following their fate through fluorescence-based assays, namely flow cytometry and imaging. These methods are often used to follow the nanocarrier internalization and targeting; however, the validity of the obtained results strictly depends on how much the nanosystem’s fate can be inferred from the fate of fluorescent dyes. To evaluate the parameters that affect the physicochemical and biological stability of the labeled nanosystems, we studied the versatility of two lipid dyes, TopFluor®-PC and Cy5-DSPE, in conventional liposomes utilizing well-defined in vitro assays. Our results suggest that the dye can affect the major characteristics of the system, such as vesicle size and zeta-potential. However, a nanocarrier can also affect the dye properties. Medium, temperature, time, fluorophore localization and its concentration, as well as their interplay, affect the outcome of tracing experiments. Therefore, an in-depth characterization of the labeled nanosystem should be fundamental to understand the conditions that validate the results within the screening process in optimization of nanocarrier. (2020-07-09)
This dataset is a collection of laboratory experiments for the characterization of the physicochemical properties of fluorescently labeled liposomes, as well as their behavior in biological environment. The experiments include: size stability (NICOMP photon correlation spectroscopy), zeta-potential stability (Zetasizer), fluorescence stability (SPARK spettrofluorimeter), biological effect (NO production on LPS-induced macrophages, cell proliferation on macrophages and keratinocytes), fluorescence tracing (Flow cytometry and imaging). (2020-09-28) |
Subject
| Chemistry; Medicine, Health and Life Sciences |
Keyword
| Liposomes (MeSH) https://www.ncbi.nlm.nih.gov/mesh/68008081
Nanomedicine (MeSH) https://www.ncbi.nlm.nih.gov/mesh/68050997
Fluorescent Dyes (MeSH) https://www.ncbi.nlm.nih.gov/mesh/68005456 |
Related Publication
| Cauzzo, J.; Nystad, M.; Holsæter, A.M.; Basnet, P.; Škalko-Basnet, N. Following the Fate of Dye-Containing Liposomes In Vitro. Int. J. Mol. Sci. 2020, 21, 4847. doi: 10.3390/ijms21144847 https://doi.org/10.3390/ijms21144847 |
Language
| English |
Producer
| UiT The Arctic University of Norway (DTD Research Group, UiT) https://en.uit.no/ |
Production Date
| 2020-04-24 |
Production Location
| Tromsø, Norway |
Contributor
| Related Person : Lyså, Roy Andre |
Funding Information
| European Commission: 766181 |
Distributor
| UiT The Arctic University of Norway (UiT The Arctic University of Norway) https://dataverse.no/dataverse/uit |
Depositor
| Cauzzo, Jennifer |
Deposit Date
| 2020-09-28 |
Date of Collection
| Start Date: 2019-01-07 ; End Date: 2019-11-22 |
Data Type
| Experimental data |