Description
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These data belong to the comprehensive study of polyphenols in Laminaria hyperborea, Laminaria digitata, Saccharina latissima, Ascophyllum nodosum and Fucus vesiculosus performed in relation with the publication titled: "Advancing Quantification methods for Polyphenols in Brown Seaweeds - applying a Selective qNMR Method compared with the TPC Assay". The dataset contains 22 files, describing the quantitative content of polyphenols in the brown seaweeds Laminaria hyperborea, Laminaria digitata, Saccharina latissima, Ascophyllum nodosum and Fucus vesiculosus. The quantitative content was determined by Optimized Total Phenolic Content (TPC) assay and quantitative NMR (qNMR). (2022-04-06)
Publication abstract: Polyphenols are important components of seaweeds contributing to their potential use in food-, feed- and health applications. In this study, optimized methods of the Folin-Ciocalteu total phenolic content assay (TPC) and selective quantitative 1H NMR (qNMR), including 13C NMR characterization, were compared for selected brown seaweeds from sublittoral to intertidal zone (Laminaria hyperborea, Laminaria digitata, and Fucus vesiculosus). TPC and selective qNMR resulted in comparable quantities for the sublittoral growing Laminariaceae, with lower polyphenolic content (TPC: 0.6–0.7%; qNMR: 0.7–0.8%, GAE, DW). F. vesiculosus, found in more shallow waters with a higher polyphenolic production, yields a TPC quantification more than double the qNMR quantification (TPC: 3.7%; qNMR: 1.5% GAE, DW). This difference most likely reflects the lack of sensitivity in the TPC assay concerning species with interfering compounds and/or a higher polyphenolic content, and conceivably also a higher diversity of polyphenols (> 1 % of biomass). From the 13C NMR characterization, L. hyperborea, L. digitata, and F. vesiculosus all showed a larger proportion of phlorethol-like phlorotannins compared to fucol-type phlorotannins. (2022-04-06)
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Keyword
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Total Phenolic Content, qNMR, Polyphenols, Laminaria hyperborea, Laminaria digitata, Fucus vesiculosus, Saccharina latissima, Ascophyllum nodosum, Quantification, TPC |