Supplemental material for: Methane category, immune response, feed efficiency, and rumen microbial community in lactating dairy cows

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Nur Cahyo, Hendra, 2026, "Supplemental material for: Methane category, immune response, feed efficiency, and rumen microbial community in lactating dairy cows", https://doi.org/10.18710/IIBITU, DataverseNO, V1
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Description
The supplementary material provides information for the article published in Journal of Dairy Science, entitled "Methane category, immune response, feed efficiency, and rumen microbial community in lactating dairy cows". This study aims to assess relationships of enteric methane (CH4) yield (g/kg of dry matter intake (DMI)) with immune response, feed efficiency (energy corrected milk (ECM)/DMI), and rumen microbiome in dairy cows, both in early and in late lactation. The DMI, body weight, ECM yield, and CH4 emission were measured in respiration chambers in early (n = 20, 32 ± 7 days in milk (DIM)) and non-pregnant late lactating (n=14, 359 ± 90 DIM) multiparous Holstein cows. The in vitro immune response was studied in response to (1) lipopolysaccharide using whole blood, and (2) phytohemagglutinin and concanavalin A using peripheral blood mononuclear cells. The DNA was extracted from rumen content samples (esophageal tubing) for 16S rRNA microbial analysis. Cows were divided retrospectively into an equal number of cows with low (LMY) and high (HMY) CH4 yield within each lactation group.
Subject Agricultural Sciences
Keyword Methane, immune response, feed efficiency, rumen microbes
Related Publication Is Supplement To: Cahyo, H. N., P. Niu, P. B. Pope, U. Gimsa, B. Kuhla, and A. Schwarm. 2026. Methane category, immune response, feed efficiency, and rumen microbial community in lactating dairy cows. Journal of Dairy Science.doi: 10.3168/jds.2025-26925
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00_README.txt
Plain Text - 15.7 KB
Published Feb 6, 2026
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MD5: 61293bfde13627884f6ef3332942f73a
The file includes information about how the dataset was created, how complete it is, and overview of the dataset.
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01_Table S1.csv
Comma Separated Values - 313 B
Published Feb 6, 2026
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MD5: f93da9030861878048d2a8a3e904d566
The file includes information about optical density from peripheral blood mononuclear cell (PBMC) assay and whole blood assay of cows with low (LMY) and high CH4-yield (HMY) in early and late lactation
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02_Table S2.csv
Comma Separated Values - 941 B
Published Feb 6, 2026
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MD5: 3e9a9ec31a8f1123724f45a6e704c8b1
The file includes information about estimated gas exchange variables and energy balance of cows with low (LMY) and high CH4-yield (HMY) in early and late lactation
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03_Table S3.csv
Comma Separated Values - 260 B
Published Feb 6, 2026
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MD5: 6c0ad7d25c3fc9025fa208ff545afad6
The file includes information about alpha diversity indices of ruminal microbiota of cows with low (LMY) and high CH4-yield (HMY) in early and late lactation
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04_Supplemental_Materials.pdf
Adobe PDF - 461.9 KB
Published Feb 6, 2026
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MD5: c8cb61638f99232534f9763e8b512154
The file contains overall information including: 1) Optical density data from peripheral blood mononuclear cell assay and whole blood assay; 2) Estimated gas exchange variables and energy balance; 3) Alpha diversity indices of ruminal microbiota; and 4) Relative abundance (%) of rumen microbial composition in phylum level between cows with low and high methane yield in early and late lactation.
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