Supplementary Data for: Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis

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Rainsford, Philip; Rylandsholm, Fredrik G.; Jakubec, Martin; Silk, Mitchell; Juskewitz, Eric; Ericson, Johanna U.; Svendsen, John S.; Engh, Richard A.; Isaksson, Johan, 2023, "Supplementary Data for: Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis", https://doi.org/10.18710/XZB5KI, DataverseNO, V1

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Description	This dataset contains raw data traces from Surface Plasmon Resonance (SPR) and Microscale Thermophoresis (MST) experiments aimed at determining the binding affinity of peptides with lipid vesicles and lipid nanodiscs. The SPR data consists of responses obtained from cycles of peptide injection over the lipid membrane, with varying peptide concentrations. The MST data includes fluorescence responses obtained from capillaries with different ratios of tested peptides and lipids. Tested hexapeptides were 1-c(WWWRRR), 2-c(WRWRWR), 3-c(WWWKKK), 4-c(WKWKWK) and 5-c(LWwNKr). The lipid compositions used in the experiments were pure 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), DMPC with 5% (w/w) 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DMPG), or DMPC with 10% (w/w) lipopolysaccharides from Escherichia coli O111:B4. These data traces are essential for measuring KP (lipid partitioning constant), understanding the peptide-lipid interaction and identifying potential candidates for further analysis. (2023-05-04)
Subject	Chemistry; Medicine, Health and Life Sciences
Keyword	SPR, Surface Plasmon Resonance, Microscale Thermophoresis, MST, Lipid nanodiscs, antimicrobials, Lipid vesicles, binding affinity
Related Publication	Rainsford P, Rylandsholm F, Jakubec M, Juskewitz E, Silk M, Engh RA, et al. Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis. ChemRxiv. Cambridge: Cambridge Open Engage; 2022 doi: 10.26434/chemrxiv-2022-hpf6f-v2
License/Data Use Agreement	CC0 1.0

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Citation Metadata

Persistent Identifier	doi:10.18710/XZB5KI
Publication Date	2023-05-09
Title	Supplementary Data for: Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis
Author	Rainsford, Philip (UiT The Arctic University of Norway) Rylandsholm, Fredrik G. (UiT The Arctic University of Norway) Jakubec, Martin (UiT The Arctic University of Norway) - ORCID: 0000-0002-6180-6900 Silk, Mitchell (UiT The Arctic University of Norway) Juskewitz, Eric (UiT The Arctic University of Norway) Ericson, Johanna U. (UiT The Arctic University of Norway) Svendsen, John S. (UiT The Arctic University of Norway) Engh, Richard A. (UiT The Arctic University of Norway) Isaksson, Johan (UiT The Arctic University of Norway)
Point of Contact	Use email button above to contact. Jakubec, Martin (UiT The Arctic University of Norway) Rainsford, Philip (UiT The Arctic University of Norway)
Description	This dataset contains raw data traces from Surface Plasmon Resonance (SPR) and Microscale Thermophoresis (MST) experiments aimed at determining the binding affinity of peptides with lipid vesicles and lipid nanodiscs. The SPR data consists of responses obtained from cycles of peptide injection over the lipid membrane, with varying peptide concentrations. The MST data includes fluorescence responses obtained from capillaries with different ratios of tested peptides and lipids. Tested hexapeptides were 1-c(WWWRRR), 2-c(WRWRWR), 3-c(WWWKKK), 4-c(WKWKWK) and 5-c(LWwNKr). The lipid compositions used in the experiments were pure 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), DMPC with 5% (w/w) 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DMPG), or DMPC with 10% (w/w) lipopolysaccharides from Escherichia coli O111:B4. These data traces are essential for measuring KP (lipid partitioning constant), understanding the peptide-lipid interaction and identifying potential candidates for further analysis. (2023-05-04)
Subject	Chemistry; Medicine, Health and Life Sciences
Keyword	SPR Surface Plasmon Resonance Microscale Thermophoresis MST Lipid nanodiscs antimicrobials Lipid vesicles binding affinity
Related Publication	Rainsford P, Rylandsholm F, Jakubec M, Juskewitz E, Silk M, Engh RA, et al. Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis. ChemRxiv. Cambridge: Cambridge Open Engage; 2022 doi: 10.26434/chemrxiv-2022-hpf6f-v2 https://doi.org/10.26434/chemrxiv-2022-hpf6f-v2
Language	English
Producer	UiT The Arctic University of Norway (UiT) https://en.uit.no/
Production Date	2022-10-01
Production Location	Tromsø
Funding Information	Research Council of Norway: DigiBiotics project - ID 269425 UiT the Arctic University of Norway: AntiBioSpec project - Cristin ID 20161326 Research Council of Norway: NanoAMP project - ID 275186
Distributor	UiT The Arctic University of Norway (UiT The Arctic University of Norway) https://dataverse.no/dataverse/uit
Depositor	Jakubec, Martin
Deposit Date	2023-05-04
Date of Collection	Start Date: 2021-09-01 ; End Date: 2022-09-30
Data Type	experimental data
Software	Biacore T200 Control and Evaluation Software, Version: 2.0 MO.Control, Version: 1.6.1
Related Material	Matlab scripts used for processing SPR files: https://github.com/MarJakubec/SPR

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